WCJC MICRO Lab 2 (BIOCHEMICAL TESTS, PROTOZOA, & HELMINTHS)

Catalase Test

What tests for the breakdown of H2O2

breakdown of H2O2

What does the Catalase Test test for?

H2O2

What is the reactant in the Catalase Test

H20 + O2(bubbles)

What is the product for the Catalase Test

Catalase

What is the enzyme in the Catalase Test

Positive= bubbles

Negative= no bubbles

What is a positive and negative reaction for the Catalase Test

Agar plate

What is the media in the Catalase Test

Denitrification test

What tests for the breakdown of nitrate

Breakdown of nitrate (NO3)

What does the Denitrification Test test for

NO3

What is the reactant in the Denitrification test

N2 (bubbles)

What is the product in the Denitrification test

Nitrate reductase

What is the enzyme in the Denitrification test

Positive= bubble in the Durham tube

Negative=no bubble

What is a positive and what is a negative reaction for the Denitrification test

Nitrate broth tube

What is the media for the Denitrification test

Durham tube

What is the small tube in in the Denitrification test

Hydrogen Sulfide (H2S) Production

What tests for the breakdown of cysteine

breakdown of cysteine

What does the Hydrogen Sulfide (H2S) Production test test for

cysteine

What is the reactant in the Hydrogen Sulfide (H2S) Production test

H2S + Fe (Blackening)

What is the product in the Hydrogen Sulfide (H2S) Production test

cysteine desulfurase

What is the enzyme tested for in the Hydrogen Sulfide (H2S) Production test

Sulfide Indole Motility Medium (SIM)

What is the media in the Hydrogen Sulfide (H2S) Production test

Positive= blackening of medium

Negative= no color change

What is a positive and negative reaction for the Hydrogen Sulfide (H2S) Production test

Indole Production Test

What tests for the breakdown of tryptophan

breakdown of tryptophan

What does the Indole Production Test test for

Tryptophan

What is the reactant in the Indole Production Test

Indole

What is the product in the Indole Production Test

Tryptophanase

What is the enzyme in the Indole Production Test

Sulfide Indole Motility Medium (SIM)

What is the media used for the Indole Production Test

Positive= red color

Negative= no color change

What is a positive and negative reaction during the Indole Production Test

Methyl Red test

What tests for many enzymes (fermentation)

many enzymes

What does the Methyl Red test test for

Glucose

What is the reactant in the Methyl Red test

Many acids

What is the product in the Methyl Red test

Positive= Many enzymes (pyruvic, acetic, formic)

Negative= ONLY pyruvic acid

What is the enzymes found in the Methyl Red test

Positive= red color

Negative= yellow color

What is a positive and negative result in the Methyl Red test

MRVP Broth

What is the media for the Methyl Red test

Oxidation-Fermentation Test

What tests for O2 requirements

O2 requirements

What does the Oxidation-Fermentation Test test for

obligate areobes (O2 needed for growth)

What does 1 green and 1 yellow tube mean

facultative anaerobes (NO O2 needed for growth)

What does 2 yellow tubes mean

Oxidation-Fermentation medium (OF)

What media does the Oxidation-Fermentation Test use

It starts green

What is special about the O-F media used in the Oxidation-Fermentation Test

To limit the O2 in our test tube

Why do we use oil in the Oxidation-Fermentation Test

single celled

heterotrophic protist

What is a Protozoa

Amoeba

What is this Protozoa

Trypanosoma

What is this Protozoa

Paramecium

What is this Protozoa

Plasmodium

What is this Protozoa

Trichomonas vaginalis

What is this Protozoa

psedopodia

What is the motility for an amoeba

Amebic dysentery

What diseases are caused by ameoba

Flagella

What is the motility for a Trypanosoma

African sleeping sickness

What diseases are caused by Trypanosoma

cilia

What is the motility of the paramecium

N/A

What is the disease caused by paramecium

non motile

What is the motility of the plasmodium

malaria

What is the disease caused by plasmodium

Flagella

What is the motility of the trichomonas vaginalis

STD

What is the disease caused by trichomonas vaginalis

A parasitic worm

What is a helminth

Kirby-Bauer Test

What test is this?

Capsule stain

What stain is this?

Gram + purple only peptoglycan

What stain is this?

Gram - has lps red

What stain is this?

pinworm

What is this?

trichinella (round worm) in skeletal muscle

What is this?

Plathyhelminthes

What is the phylum for a flatworm

Nematoda

What is the phylum for a roundworm

tapeworm

What is this?

Scolex and attachment

What is the head called and what is it used for

Ascaris

What is this?

This test shows antibiotic resistance

What does a Kirby Bauer test show

broad spectrum (notice two rings)

What is this result

narrow spectrum (notice only one ring)

What is this result

Zone of inhibition

What is the ring called?

Mueller-Hinton agar

What agar is used for a Kirby Bauer test?

protein

For the yogurt test, what is stained red?

bacteria

For the yogurt test, what is stained purple?

It is both selective and differential

What is special about MacConkey Agar

Non-lactose fermenters

What does the yellow show on MacConkey Agar

Lactose fermenters

What does the pink show on MacConkey Agar

bacti-spreader tool

What is this

4 steps

how many steps are in staining

primary stain crystal violet (60 seconds)

What is the first step in staining and what does it do

Mordant iodine (60 seconds)

What is the second step in staining and what does it do

Decolorizer ethanol (30 seconds)

What is the third step in staining and what does it do

counter/ secondary stain safranin (60 seconds)

What is the fourth step in staining and what does it do

Rinse with water till water runs clear

what do you do in between each staining step

only use one dye

used to determine size, shape and arrangement

What is a simple stain

use two dyes

used to determine cell type and structure

What is a differential stain

inoculating loop on agar

What tool and media is used for a catalase test

inoculating loop in nitrate broth tube

What tool and media is used for a denitrification test

Inoculating needle in SIM

What tool and media is used for a H2S test

inoculating needle in SIM

What tool and media is used for an Indole test

inoculating needle in MR-VP

What tool and media is used for a methyl red test

inoculating needle in o/f media

What tool and media is used for an O/F test

H2O2 (hydrogen peroxide)

What do we add to catalase tests

Kovac's reagent

what do we add to indole production tests

Methyl red reagent

What do we add to methyl red tests

heat fixing

What do you do before the staining process