chapter 6 immunology test 3

generation of B cell repertoire

somatic recombination, the diversity of specificity within the BCR population

negative selection

gets rid of the cell that recognizes you through apoptosis

negative selection for B cells takes place where

bone marrow

where does maturation of B cell happen

bone marrow

where does the final maturation step of the B cell take place

organize secondary lymphoid tissue

B cells search for antigen on what cells

FDCs

B cells will bind to FDCs and then find what kind of effector T cell

Th2 to make IgE. T follicular helpers are also found too

what cell can also activate a B cell

Th1

organs of teh secondary lymphoid tissues

peyer’s patches, lymph nodes, spleen

when D and J rearrangement takes place, what is the cell called

early pro-B cell

when V joins D and J, what is the B cell called

late pro-B cell

functional rearrangement of V, D, J is what cell (no light chain)

large pre-B cell. need a surrogate light chain

3 characteristics of the surrogate light chains

1. made only in pre-B cells

2. VpreB surrogates for the VL

3. Lambda 5 surrogates for the CL

Pre-B cell receptor molecules eventually lead to

allelic exclusion

allelic exclusion can result in

transcription of RAG’s stopping and RAG-2 being tagged for destruction

randomness in gene rearragement can result in

some B cell receptors being reactive against self components

in a stem cell factor, which cell is each adhesion molecule or each signaling molecule on

stromal cells and HSCs

what do stromal cells release

IL-7 cytokines which bind to IL-7 receptor

unproductive rearrangement

stop codon being put in randomly

IgM has the highest…

avidity

BCL-2

another proto-oncogene

5% of our b cells are

b-1s

do B-2 cells use somatic recombination

yes

b-1 secretes what isotype

IgM

what surrogates for the variable region domain

VpreB

what surrogates for the constant domain in the light chain

lambda 5

what is needed for surrogate light chain

Ig alpha and Ig beta

what B cells are considered unresponsive or anergic to the antigen

ones that bind soluble self-antigens (monovalent self-antigens)

multivalent self antigens can drive..

apoptosis of the b cell

if receptor editing deosnt work, what happens

apoptosis

anergy

death by neglect

receptor editing entails

new L chain combines with the previous H chain to form a new receptor

continued synthesis of RAG proteins allows for

light chain rearrangement

CCl19 and 21 do what

they attract B cells into lymph node after CCl21 attracts immature B cells to HEV

which chemokine directs the B cells to the follicle from the T cell area of the lymph node

CXCL13

CCL19 and 21 bind to

CCR7

CXCL13 binds to what

CCR5

final activation signal for B cell

BAFF

FDCs make

BAFF