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Carbohydrates, lipids, proteins, nucleic acids, ATP, water and inorganic ions
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What are the key molecules that are required to build structures that enable organisms to function?
Carbohydrates
Proteins
Lipids
Nucleic acids
Water
What is a monomer?
Smaller units from which larger molecules are made
What is a polymer?
Molecules made from large numbers of monomers joined together in a chain during a process called polymerisation
What are two important reactions that occur to form covalent bonds in molecules?
Condensation reactions
Hydrolysis reactions
What is a condensation reaction?
A reaction that occurs when monomers combine by covalent bonds to form polymers or macromolecules, and water is removed
What does hydrolysis mean?
“lyse” = to break
“hydro” = with water
What happens in a hydrolysis reaction?
In the hydrolysis of polymers, covalent bonds are broken when water is added.
What is a monosacharride?
A single reducing sugar monomer
What elements do all carbohydrates contain?
Carbon
Hydrogen
Oxygen
What are some examples of monosacharrides?
Glucose
Fructose
Deoxyribose
What do monosacharrides join together to form?
Disacharrides
Polysacharrides
How can sugars be classified and why?
Reducing
Non-reducing
Classification into these groups is dependent on the sugars ability to donate electrons
Can reducing sugars donate electrons?
yes
Can non-reducing sugars donate electrons?
No
How do you test for reducing sugars?
Add benedict’s solution and heat
blue > green > yellow > orange > brick red
What are some examples of reducing sugars?
Glucose
Fructose
Galactose
Fructose and galactose have the same molecular formula as glucose however, they have a different structural formula
How do you test for non-reducing sugars?
1) Test using benedict’s solution - if negative…
2) Boil with concentrated acid to hydrolyse the glycosidic bond
3) Neutralise the acid with a base
4) Test again with benedict’s solution and heat - if positive, non-reducing sugars are present… if negative again, there is no sugar at all.
What is an xample of a non-reducing sugar?
Sucrose
What are the two forms of glucose?
Alpha
Beta
Where is the hydroxyl (OH) groups on carbon 1 located in alpha glucose?
Below the ring
Where is the hydroxyl (OH) groups on carbon 1 located in beta glucose?
Above the ring
What is a disacharride?
Two monosaccharides joined together with a glycosidic bond with a biproduct of water
What is a polysaccharide?
Many monosaccharides joined together with a glycosidic bond with a biproduct of water
What are 3 examples of polysaccharides?
Starch
Glycogen
Cellulose
What type of glucose is starch made from?
Alpha glucose
What type of glucose is glycogen made from?
Alpha glucose
What type of glucose is cellulose made from?
Beta glucose
How are disaccharides and pilysaccharides formed?
When two hydroxyl (-OH) groups (on different saccharides) interact to create a strong covalent bond called the glycosidic bond
What is maltose made up of?
2 alpha glucose molecules
What is sucrose made up of?
An alpha glucose molecule and a fructose molecule
What is lactose made up of?
An alpha glucose molecule and a galactose molecule
How is a glycosidic bond broken?
When water is added in a hydrolysis reaction
What are some examples of disaccharides?
Maltose
Sucrose
Lactose
What is starch?
The storage polysaccharide of plants
How is starch stored?
As granules in plastids (e.g. chloroplasts)
Why does starch take longer than glucose to be digested?
Because there are many monomers in it
What 2 polysaccharides is starch constructed from?
Amylose
Amylopectin
What is glycogen?
The storage polysaccharide in animals and fungi
What is the structure of glycogen?
Highly branched structure
What does glycogen’s highly branched structure do?
Makes glycogen more compacts, which helps animals to store ore of it
Branching enables more free ends where glucose molecules can be added or removed allowing for condensation and hydrolysis reactions to occur more rapidly.
Where is cellulose found?
In plant cells
What gives cellulose its strength?
The inversion of beta-glucose molecules means that many hydrogen bonds form between the parallel chains of microfibrils
What is the main function of cellulose?
It is the main structural component of cell walls
What is a microfibril?
A very fine fibril, or fibre-like strand, consisting of glycoproteins and cellulose
What is good about the strangth of cellulose?
The high tensile strength of cellulose allows it to be stretched without breaking, which makes it possible for cell walls to withstand turgor pressure
Are cellulose fibres permeable?
Yes, Cellulose fibres are freely permeable, which allows water and solutes to leave or reach the cell surface membrane
How do you test for starch?
1) Add a few drops of iodine solution
2) If starch is present iodine with go from orange/brown to blue/black