Microscopy Quiz (AP)

Microscope Parts and Functions, Types of Microscopes

Ocular Lens

Body Tube

Base

Coarse Adjustment Focusing Knob

Fine Adjustment Focusing Knob

Arm

Revolving Nosepiece

Objective Lenses

Stage Clips

Stage

Condenser

Diaphragm

Light/Lamp

Base

Supports the Microscope

Arm

Supports the body tube and lenses and connects it to the base

Body Tube

Connects ocular lens to objective lenses; pathway light follows from specimen to eye

Coarse Adjustment Focusing Knob

Puts specimen into a general focus, moves the stage big distances

Fine Adjustment Focusing Knob

Brings specimen into detailed focus, moves the stage tiny amounts

Objective Lenses

Low Power - 4X

Medium Power - 10X

High Power - 40X

Oil Immersion - 100X

Ocular Lens

Lens that you look through, magnifies the image 10X

Stage

Flat platform for the slide

Stage Clips

Keeps slide in place

Revolving Nosepiece

Holds objective lenses; can be rotated

Diaphragm

Controls the amount of light going through the specimen

Condenser

Focuses light before it passes through the specimen

Compound Microscope

• light rays pass through the specimen

• Views internal structures of small organisms

• Can view living specimen

• Limit of resolution: 1/5000mm

Dissecting Microscope

• Light rays bounce off the specimen

• Views external structures of moderately sized organisms

• Can view living cells and organisms

• Limit of resolution: 1/5000mm

TEM (Transmission Electron Microscope)

• beams of electrons pass through the specimen

• Can view very tiny internal structures

• Limit of resolution: 7 × 10^-7

SEM (Scanning Electron Microscope)

• beams of electrons bounce off the specimen

• Can view very tiny external structures

• Limit of Resolution: 7 × 10^-7

Scanning Probe Microscope

• does not use lenses

• Images use a computer to record information