Part 7: WBC

Nuclear Chromatin Pattern

On determining WBC maturity, most valuable and reliable criterion is:

Lymphocye

What is the only WBC that is not phagocyte?

Myeloblast

Earliest recognizable granulocytic precursor using the light microscope

primary (azurophilic/ nonspecific) granules

What type of granules is present in Type II myeloblasts?

promyelocyte

The rule in granulocytic series states that “overall cell size decreases” with the exemption of ______ with a size 16 to 25 um

Promyelocyte

Cytoplasm is evenly basophilic and full of primary (azurophilic) granules

Hof

Hollow in the cytoplasm of a cell that lodges the nucleus

Myelocyte

Last stage capable of mitosis

Myelocyte

Stage of synthesis of secondary granules (aka specific granules)

Dawn of Neutrophilia

To differentiate early myelocyte vs late myelocyte, look for ____________

4.3 days

No. of days myelocyte becomes metamyelocyte

15 hours

Length of time myeloblast becomes promyelocyte

24 hrs

Length of time promyelocyte becomes myelocyte

Metamyelocyte

aka Juvenile cell; first stage of nuclear identification

Metamyelocyte

Synthesis of tertiary granules (aka gelatinase granules)

Band cell

aka Stab cell or staff cell; youngest granulocytic precursor to normally appear in the peripheral blood

<0.5 × 10⁹ / L

Agranulocytosis is neutrophil count that is _______________

myeloblast

It has scanty cytoplasm

amidopyrine and cephalosporin

Associated drugs with extreme neutropenia

Trichinosis

It produced the highest eosinophil count

5

How many precursors are there in GRANULOCYTE SERIES?

2

How many precursors are there in AGRANULOCYTE SERIES?

Small lymohocyte

Is the predominant form of lymphocyte in the blood with size 6 to 9 um

Plasma cells

Final maturation stage of B lymphocytes

Plasma cell

Has a tortoise shell or clockface in appearance

Plasma cell

Cytoplasm is composed of discrete globules called Russel Bodies

Plasma Cell Myeloma

Most common malignant disease of plasma cells

Macrophages

Most abundant cell type in the body

Transcobalamin II

The primary transport factor for Vitamin B12

holoTC

Metabolically active form of Vitamin B12.

Also called the Vit B12 transcobalamin complex

Kupffer cells

Macrophage in the liver

Dust cell/ Alveolar macrophage

Macrophage in the lungs

Mesangial cells

Macrophage in the kidneys

Microglial cells

Macrophage in the brain

Langerhans cells

Macrophage in the skin

Littoral cells

Macrophage in the spleen

Type A cells

Macrophage in the synovial tissue

Osteoclast

Macrophage in the bone

Hoffbauer cells

Macrophage in the placenta

Dendritic cells

Macrophage in the lymph nodes

7 hrs

Half-life of neutrophils in the blood

G-CSF

Major stimulatory cytokine for neutrophil production

GMP

Neutrophils and monocytes are direct descendants of a common progenitor known as:

Neutrophil

What cell produces Transcobalamin I?

Macrophage

What cell produces Transcobalamin II?

MPO

An enzyme that exhibits bactericidal, fungicidal, and viricidal properties

Flow cytometry and cytochemical staining

Used to detect MPO in myeloid precursors in acute leukemia

Lysozyme

aka muramidase, an antimicrobial enzyme

Lactoferrin

An iron-binding protein that competes with bacteria for iron

Azurophilic

Primary granules is also known as

Specific

Secondary granules is also known as

Gelatinase

Tertiary granules is also known as

CD 11b/ CD18

Contribute to tight stationary binding between neutrophils and endothelial cells

Alkaline phosphatase

The only WBC that has Alkaline phosphatase activity in Neutrophils

C

Which of the ff granules can gelatinase be found?

a. primary

b. secondary

c. both a and b

d. AOTA

B

Which granule can lactoferrin be found?

a. primary

b. secondary

c. tertiary

d. aota

Hematopoietic Stem Cell

The lone cells in Stem cell pool

18 hours

Circulating half-life of eosinophils

2 to 5 days

Survival time of eosinophils in human tissues

Black

Color of Charcot-Leyden in Hematoxylin

Colorless

Normal Color of Charcot-Leyden

Red

Color of Charcot-Leyden in Eosin

Purplish-Red

Color of Charcot-Leyden in Trichrome Stain

Lipid bodies

In which of the ff eosinophilic granules can one find cyclooxygenase?

a. Primary granules

b. Secondary granules

c. Small lysosomal granules

d. lipid bodies

e. storage (secretory) vesicles

Secondary Granules

In which of the ff eosinophilic granules can one find Major Basic Protein?

a. Primary granules

b. Secondary granules

c. Small lysosomal granules

d. lipid bodies

e. storage (secretory) vesicles

60 hours

Life span of basophils

Toluidine Blue

Stain for Basophils, it is a dye that can bind with acid mucopolysaccharides in blood cells to form metachromatic complexes

Basophil

Least common WBC in normal peripheral blood

Monoycte

Round, horse-shoe or lobulated nucleus with ground-glass appearance cytoplasm

NSE (Nonspecific Esterase) Stain

Stain used for monocytes

Azure dust

Indistinct granules found on monocytes is called

3 days

Monocytes remain in the circulation roughly:

Lymphocyte

Sky blue or “Robin egg” blue

Variant lymphocyte

aka Downey cells/ glandular fever cells

Type I

What type of Variant Lymphocyte is seen in German Measles

Type II

What type of Variant Lymphocyte IS Infectious Mononucleosis Cell found?

Type II

What type of Variant Lymphocyte is characterized by “flared skirt” or “fried egg”

1% ammonium oxalate

1% Hcl

3% acetic acid

Diluting fluid of WBC

1% ammonium oxalate

Diluting fluid of platelets

Isotonic saline

Diluting fluid of RBCs

NRBCs

What is not lysed by the WBC diluting fluid?

1:20

Most common dilution for WBC

1:100

Dilution for Plt and RBCs

10 minutes

In WBC count procedure, allow the dilution to sit for _______ to ensure that the RBCs have lysed

3 hours

WBC counts should be performed within ________ of dilution

10 minutes

How many minutes is the charged hemocytometer placed in a moist chamber?

PMF (Primary Myelofibrosis)

Leukoerythroblastic reaction is strongly associated with

Volume, Conductivity, Scatter

VCS in WBC differential stands for

Multi-angle Polarized Scatter Separation

MAAPS in WBC differential stands for