MICRO FINAL pt II

Genus

____________ is the level of classification that divides families into several genera’s. 

Species

____________ comes after genus in classification and is the most **specific** level of orginization

endospores

_____________ are inert(cant move) **resting** cells produced by some G+ bacteria such as Clostridium and Bacillus

coccus

bacillus

spirillum

what are the 3 basic shapes of bacteria

A. Liquid

broth is a ____________

A. Liquid

B. Solid

B. Solid

Agar is a ____________ derived from seaweed

A. Liquid

B. Solid

True

T/F broth does not contain agar

Macconkey agar

____________ is the best media to identify causative agent of UTI(E.coli)?

C. Both

Manitol Salt Agar (MSA) is _________

A. Selective

B. Differential

C. Both

C. Both

Manitol Salt Agar is ___________

A. Selective

B. Differential

C. Both

A. Selective

Eosine Methylene Blue Agar is ___________ to gram negative bacilli/rods

A. Selective

B. Differential

C. Both

B. Differential

Blood Agar Media is __________ and enriched

A. Selective

B. Differential

C. Both

Mannitol salt Agar (MSA)

MacConkey Agar (MAC)

Eosin Methylene Blue Agar (EMB)

Blood Agar (BA)

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what are the 4 most common medias used in medical and diagnostic labs

D. Blood Agar (BA)

______________ media contains 5% sheep’s blood and is a enriched differential media that can differentiate Streptococci

A. Mannitol salt Agar (MSA)

B. MacConkey Agar (MAC)

C. Eosin Methylene Blue Agar (EMB)

D. Blood Agar (BA)

selective

____________ media allows only certain organisms to grow while inhibiting the growth of others. 

differential

______________ media are used to differentiate between different organisms as it allows the groth of more than one organism

True

T/F some media can be both selective and differential

Manitol Salt Agar

___________ contains 7.5% of NaCl and is used for isolation of Staphylocci.

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differential

Mannitol Salt Agar __________ factor is phenol red that turn yellow in the presence of acid

A. Selective

B. Differential

aureus

Staphylococcus _________ ferments mannitol agar leading to a yellow color

A. epidermis

B. aureus

A. epidermis

Staphylococcus __________ grows on MSA but does not ferment the mannitol

A. epidermis

B. aureus

MAC

_____________ is a selective medium used for isolation of Gram-negative bacteria. 

A. Mannitol salt Agar (MSA)

B. MacConkey Agar (MAC)

C. Eosin Methylene Blue Agar (EMB)

D. Blood Agar (BA)

A. Selective

Crystal violet and bile salts is the __________ in MacConkey agar (MAC)

A. Selective

B. Differential

B. Differential

Lactose is the _________ factor in MAC

A. Selective

B. Differential

A. *Escherichia coli (E. Coli)*

______________ can grow on MAC media

A. *Escherichia coli (E. Coli)*

B. Staphylococcus epidermis (*S. epidermis*)

C. Staphylococcus aureus (*S. aureus*)

A. Selective

*Eosin and methylene blue, bile:* is the __________ factor is EMB that Inhibits the growth of most Gram-positive organisms. 

A. Selective

B. Differential

A. Streptococci

blood agar is used to differentiate

A. Streptococci

B. Staphylococci

A. Purple

A gram positive stain turns _______

A. Purple

B Pink

B. Pink

A gram negative stain turns ____________

A. Purple

B. Pink

sheep

the deferential factor in Blood Agar is ___________ erythrocytes

B. Alpha Hemolysis

_________________ is the complete lysis of sheep erythrocytes

A. Beta hemolysis

B. Alpha Hemolysis

C. Gamma Hemolysis

A. Beta hemolysis

_________________ is the partial lysis of sheep erythrocytes

A. Beta hemolysis

B. Alpha Hemolysis

C. Gamma Hemolysis

C. Gamma Hemolysis

______________ is the no lysis of sheep erythrocyte

A. Beta hemolysis

B. Alpha Hemolysis

C. Gamma Hemolysis

synthetic

____________ media precisely defined (we know what the media consists of)

general

_______________ media contains complex organic substances (ex. blood)

complex

______________ media is undefined media that contains complex unknow/unmeasurable amount of substances

negative

Escherichia coli (E. Coli) is gram __________ rod

Escherichia coli (E. Coli)

_____________ bacteria can be found in the intestine and rectum

B. Clusters

what is the arrangement of Staphylococcus (epidermis, aureus)

A. Chains

B. Clusters

A. Chains

what is the arrangement of streptococcus

A. Chains

B. Clusters

pyogenes

streptococcus _________ is the causative agent of strep throat

Nucleus

The _____________ is the genetic center of the cell and building/storage center for DNA and synthesis of RNA

nucleolus

Ribosomal RNA and ribosome construction happens in the ____________

lipid

the Endoplasmic reticulum (ER) transport material and is the place for _______ synthesis

Golgi apparatus

packaging and modification of protien synthesis prior to secretion happens in the ____________

lysosomes

___________ are membrane bound organelle that contains digestive enzymes and helps with intracellular digestion

osmotic

Vacuoles temporarily store and transport it is also a organelle that regulates _________ pressure

mitochondria

the power house of the cell the produces ATP is known as the ___________

Chloroplast

_________ converts sunlight energy into chemical energy through photosynthesis

protien

Ribosomes are responsible for __________ synthesis

cytoskeleton

______________ is made up of microfilaments and microtubules and provides cell with structure and movement

True

T/F viruses contain nucleic acid

False (prokaryotes do this is where they assemble genetic material since they don’t have a nucleus)

T/F eukaryotes have nucleoids

organelles

_______________ are structures specialized to preform distinct processes within a cell

can cause thrush

responsible for vaginal yeast infection

is a fungus

name 3 characteristics of Candida albicans

fungus

mycosis is any disease caused by a _____________

Malassezia

Tinea versicolor (skin/scalp infection) is caused by ______________ furfur

trichomoniasis (T homilis)

___________ is a STD caused by a single cell protozoan and is the most common type of STD

protzoan

a unicellular eukaryotic organism is called a ______________

plasmodium

malaria is caused by a single cell protozoan parasite called _______________