plasmids ch 5

transformation

A process where bacteria pick up DNA from environment is referred to as

shuttle

The classification of a plasmid that can be used to infect eukaryotic cells is a(n)

transformation efficiency

This value is measured in CFU/micro gram

UV spectrophotometer

device that uses light to quantify plasmid DNA in solution

gDNA

The the large chromosome of an E coli is classified as this

Transduction

The process of transfer of DNA through a viral phage vector to a bacterium is called

constitutive

A gene that is always turned on in bacteria to provide for normal function is referred to as

heat shock

A step, in the transformation process, to make them more competent by expanding the pore spaces in the membrane is referred to as

DNA polymerase

The enzyme that copies DNA is

facultative

a gene classification, in bacteria, indicating that is only transcribed when needed

centrifugation

 This is the process of spinning a sample to separate by mass

cloning vector

A plasmid that carries a gene that is to be amplified by bacteria is know as a(n)

plasmid

A small circular piece of DNA transferred between bacteria

F+

What an E coli must be to initiate conjugation

AUG

The universal start codon in DNA

biolistics

The process of gene insertion that uses gold or tungston fragments to shoot DNA into the host

spin, remove the supernatant, lysis, spin, pH neutralization, spin, binding, spin, wash, elution

The proper sequence of miniprep, starting with a LB broth culture, is best represented by

aseptic

The type of technique that resists cross contamination

expression vector

A plasmid that is used to produce viable protein products from E coli is know as a(n)

electroporation

The process of making a cell membrane more competent to uptake DNA

inducer

binds to the repressor to inactivate it

Hind III

This enzyme cuts DNA at a specific palindrome location

AMPr

gene that allows E coli to grow on an antibiotic plate

ORI

The site on a plasmid where DNA polymerase can attach is know as the

competent

type of bacteria that is ready to receive a plasmid

promoter

The region on a plasmid where RNA polymerase attaches is the

is used as a selectable marker

A gene that allows bacteria to glow green

2

Number of different restriction sites recommended for proper insertion of a gene into a plasmid

repressor

This molecule binds to the operon to hide the promotor region

37C

best growth temperature for E coli

alcohol

Substance is added to remove salts during the miniprep in the process of plasmid precipitation

arabinose

The sugar that acts as the inducer in the araBAD operon

conjugation

The process where bacteria directly transfer DNA to each other through a cytoplasmic bridge is called

lethal

The type of gene that can be inserted into a plasmid that kills the bacteria if a gene is not properly inserted into the MCS site

operon

A control unit in bacteria to turn and off a series of genes

miniprep

Name of the process of purifying and amplifying plasmids from transformed bacteria

terminator

A sequence on a plasmid that signals the end of transcription

CaCL2

chemical used in chemical electroporation to make the plasma membrane competent

UV

Light with a wavelength of 260nm is absorbed by plasmid DNA. This wavelength is classified as

fluorescent

Selective marker type that was activated by arabinose pGLO plasmid

DNA ladder (bp)

type of ruler used in gel quantification

alkiline

This type of lysis buffer is added to denature the plasma membrane during miniprep

ice, plasmids, ice for 10min, heat 45C for 45s, ice 2min, LB broth 10min at 37C, plate, incubate at 37C

What is the correct sequence in the transformation of E.coli starting with a cold tube containing CaCl2 and competent cells

vessicles

tiny lipid sphere made of membrane material used in lipid transfection

upstream

location of a gene in reference to the promoter

give antibiotic resistance

bla is a gene used in plasmids to

vector

Something that transfers DNA from the reservoir to the host is classified as a

supernatant

The liquid material above the pellet after centrifugation is called the

MCS site

A site in an engineered plasmid that contains a string of restriction sites is a(n)

E.Coli

The cloning host most often used in biotechnology