Biotechnology

studied byStudied by 2 people
0.0(0)
learn
LearnA personalized and smart learning plan
exam
Practice TestTake a test on your terms and definitions
spaced repetition
Spaced RepetitionScientifically backed study method
heart puzzle
Matching GameHow quick can you match all your cards?
flashcards
FlashcardsStudy terms and definitions

1 / 16

encourage image

There's no tags or description

Looks like no one added any tags here yet for you.

17 Terms

1

Recombinant DNA Technology

Process of combining DNA from different sources to produce artificial recombinant DNA

New cards
2

Recombinant DNA

Artificial DNA formed by combining DNA from different sources

New cards
3

Genetically Modified Organisms

Organisms with altered DNA

New cards
4

Transgenic Organisms

Organisms that have had their DNA altered with foreign DNA from different species

New cards
5

Process

  1. Identify desired gene

  2. Restriction enzyme cuts DNA & isolates gene

  3. Same restriction enzyme cuts vector

  4. Splice gene with vector

  5. DNA ligase bind sticky ends

  6. Form recombinant DNA

  7. Insert into host cell

  8. Cultured + cloned

  9. Extracted & purified

New cards
6

Restriction Enzymes

Enzymes that cut DNA at specific sequences of nucleotides

New cards
7

Recognition Site

Specific sequence of nucleotides where enzyme cuts DNA

New cards
8

Palindromic

Sequences that reads same forwards & backwards

New cards
9

Straight Cut

Restriction enzyme makes clean break across 2 DNA strands to produce blunt ends

New cards
10

Blunt Ends

DNA fragments with paired base endings produced by straight cuts

New cards
11

Staggered Cut

Restriction enzyme cuts DNA at different locations to produce sticky ends

New cards
12

Sticky Ends

DNA fragments forming overhangs with unpaired base pairings produced by a staggered cut

New cards
13

DNA Ligase

Enzyme capable of combining 2 single stranded DNA segments into 1 structure

New cards
14

DNA Ligase Process

  • Complementary bases MUST form hydrogen bonds

  • Bind phosphate group to sugar molecule

New cards
15

Vectors

Bacterial plasmids/viral phages used to transfer DNA into a cell

New cards
16

Plasmid

Small circular strand of cytoplasmic DNA in bacteria that can replicate independently

New cards
17

Bacteriophages

Viruses that infect bacteria

New cards
robot