A&P1 Chapter 2

Ocular (Eyepiece)

The lens you look through; typically provides 10x magnification.

Objective Lens

Lens closest to the specimen; provides magnification ranging from 4x to 100x.

Nosepiece

Holds the objective lenses and rotates to change magnification.

Stage

Flat platform where the slide is placed.

Coarse Adjustment Knob

Moves the stage up and down for general focusing.

Fine Adjustment Knob

Fine-tunes the focus and increases the detail of the specimen.

Condenser

Focuses light onto the specimen.

Iris Diaphragm

Controls the amount of light reaching the specimen.

Base

Supports the microscope and houses the light source.

Light Source

Provides light to view the specimen.

Total Magnification

Calculated by multiplying ocular lens power by objective lens power.

Resolution

The ability to distinguish two close objects as separate.

Oil Immersion

Technique that uses oil to improve resolution at 100x objective magnification.

What is the total magnification using a 10x ocular and 40x objective?

400x

Which part of the microscope adjusts light intensity?

Iris Diaphragm

What part should you use to clean the microscope lenses?

Lens Paper

What kind of specimen is best viewed with a stereomicroscope?

Large, opaque, 3D specimens

Why is oil immersion used?

To increase resolution by reducing light refraction.

What type of microscope produces a 3D image of the surface?

Scanning Electron Microscope (SEM)

Which type of microscopy is best for viewing live, unstained specimens?

Phase-contrast microscopy