Sanger Sequencing and Its Applications (recording)

These flashcards cover essential concepts regarding Sanger sequencing, its techniques, uses, and implications in gene sequencing.

What is Sanger sequencing known as?

It is also called chain termination sequencing or dideoxy nucleotide sequencing.

Who developed the Sanger sequencing method?

Frederick Sanger developed it in the 1970s.

What is the primary use of Sanger sequencing?

It is primarily used for sequencing one gene or for sequence verification.

What does the term 'resolution' refer to in scientific terms?

Resolution refers to the ability to distinguish small differences between two entities.

Why are dideoxynucleotides important in Sanger sequencing?

They lack a 3' hydroxyl group, causing DNA synthesis to terminate when incorporated.

What technique is used to separate DNA fragments in Sanger sequencing?

Gel electrophoresis is used to separate DNA fragments based on size.

What does an autoradiogram show in the context of DNA sequencing?

It shows the banding pattern of DNA fragments that indicates the sequence of nucleotides.

What is cycle sequencing and how does it improve the sequencing process?

Cycle sequencing increases efficiency and allows for less template DNA to be needed.

What is the maximum reliable length of sequence that can be obtained using Sanger sequencing?

The maximum reliable length is around 500 to 700 bases.

What is primer walking in DNA sequencing?

Primer walking is a method used to sequence longer DNA stretches by designing new primers from previously obtained sequences.