Microbiology: Bacterial Identification and Testing Techniques

Vocabulary practice flashcards covering bacterial identification patterns, enzymatic test results, and TSI slant characteristics based on the lecture notes.

Gram positive in chains

Strep or enterococcus

Gram negative diplococci

Neisseria spp.

Gram negative rods

Enterobacteriaceae or pseudomonas

Catalase Positive

staph

Catalase Negative

strep

Coagulase Positive

staph a.

Coagulase Negative staph

epidermidis, saprophyticus, haemolyticus

Oxidase Positive

pseudomonas or neisseria

Oxidase Negative

Enterobacteriaceae (E. coli, Salmonel la, Klebsiel la)

Novobiocin sensitive

s. epidermidis (skin contaminant)

Novobiocin resistant

s. saprophyticus (UTI in young females)

Bacitracin sensitive

Group A strep (strep. pyogenes)

Bacitracin resistant

Group B strep (strep. agalactiae)

Optochin sensitive

Strep. pneumoniae

Optochin resistant

Viridans group strep

Gamma hemolysis confirmation tests

Enterococcus spp. confirm with bile esculin and $6.5\,\% \text{NaCl}$

TSI slant Yellow

acid

TSI slant Red

alkaline

TSI slant Black precipitate

$H_2S$ production

E.coli TSI slant (slant, butt, gas, H2S)

A A + -

K. pneumoniae TSI slant (slant, butt, gas, H2S)

A A + -

Shigella spp. TSI slant (slant, butt, gas, H2S)

K A - -

Salmonella spp. TSI slant (slant, butt, gas, H2S)

K A + +

P. aeruginosa TSI slant (slant, butt, gas, H2S)

K K - -

IMViC acronym

I- Indole, M-Methyl red, V- Vogues-proskauer, C-Citrate

E.Coli IMViC

++--

Enterobacter aerogenes IMViC

--++

PYR test

used to rapidly identify strep. pyogenes

CAMP test

Identify Strep. agalactiae via Arrow-head formation

Urease test

used to identify Proteus