BIO120 SCIENTIFIC METHOD + MICROSCOPE

what are the 5 steps of a scientific method

observation, hypothesis, data collection, analysis of data, and conclusion

hypothesis

unproven conclusion in the form of a statement or guess. must be testable and based on facts

experiments

procedure used to describe cause and effect

theory

hypothesis validated many different times

biological principle

theory that is verified and used widely

base

support

substage light

source of light

stage

platform for slides

mechanical stage

holds the slide

mechanical stage controls

precise movement of the specimen along the x and y axis

condenser

provide the right amount of light on the specimen

condenser knob

adjusts the height of the condenser

iris diaphragm lever

regulates amount of light coming through the condenser

coarse adjustment knob

gross focusing

fine adjusting knob

precise focusing

head

holds the objective + ocular lenses

frame/arm

support of head + base

ocular lens

eyepiece, 10X magnification and the pointer is found, virtual image

nose piece

rotating piece that holds objective lenses

objective lenses

4X (scanning lens, red), 10X (low power, yellow), 40X (high power, blue), real image

resolution

ability to discriminate two close objects as separate

depth of field

depth at which the specimen is clearly in focus

working distance

distance from the bottom of the specimen to the objective lens

repeatability

the primary basis for support or rejection of many hypotheses