8.2 - BLOOD DONATION PART 2

Volume To Collect = (donor’s weight in kg/50) x 450 mL

Volume To Collect / 450 x 63 mL = reduced volume of anticoagulant

If underweight (2 formulas)

Apheresis

Collection of a specific blood component while returning the remaining whole blood components back to the patient

Based on differences in density

Greek word “aphairesis” meaning to separate or remove

Citrate

Anticoagulant for apheresis

Intermittent flow centrifugation

Continuous flow centrifugation

Methods of Centrifugation (2)

Intermittent flow centrifugation

Method of Centrifugation

Blood is processed in batches or cycles

Continuous flow centrifugation

Method of Centrifugation

Blood withdrawal, processing, reinfusion are performed simultaneously in an ongoing manner

Plasmapheresis

What procedure?

Component removed: Plasma

Plateletpheresis

What procedure?

Component removed: Platelets

Leukapheresis

What procedure?

Component removed: Leukocytes

Erythrocytapheresis

What procedure?

Component removed: RBC

HPC Apheresis

What procedure?

Component removed: Hematopoietic progenitor cells (HPC)

6-8

Plateletpheresis

When you collect platelets from plateletpheresis, the equivalent amount of the platelet harvested is equal to _-_ Random Donor Platelets

Hydroxyethyl Starch (HES)

Leukapheresis

Common sedimenting agent that enhances the separation of WBC from RBC

Corticosteroids (Prednisone or Dexamethasone)

Leukapheresis

These drugs are given to the donor prior to the collection procedure

Growth Factors

Leukapheresis

They work by pulling the granulocytes from the marginal pool into the general circulation, thus increasing the amount of cells in the circulation

Double RBC Apheresis

Double units of RBC

16 weeks

Frequency of donation: 2RBC

Every 2 days (no more than two times in 7 days)

Frequency of donation: Plasma (frequent)

Every 4 weeks (no more than 13 times/year)

Frequency of donation: Plasma (infrequent)

Every 2 days (no more than 2 times in 7 days; no more than 24 times in 12 months)

Frequency of donation: Platelets, single apheresis unit

Every 7 days

Frequency of donation: Platelets, double or triple apheresis unit

Every 2 days

Frequency of donation: Granulocytes

130 lbs

5’1”

FDA Guidelines for 2RBC: Male

Weight and min height

150 lbs

5’5

FDA Guidelines for 2RBC: Female

Weight and min height

40%

FDA Guidelines for 2RBC: Hematocrit

Infrequent Donor

Serial Donor

Plasmapheresis types of donors (2)

Infrequent Donor

Plasmapheresis

Depends on the frequency

Occasional

Undergoes no more than 1 procedure in 4 weeks period/once in a month

Serial Donor

Plasmapheresis

May donate more frequently, but no more than every 48 hours and no more than 2 donations in a 7-day period

In a 7 day period, its interval is 2 days

Therapeutic Apheresis

involves the removal of specific blood components

Therapeutic Apheresis

The rationale of _ _ is based on the following:

A pathogenic substance exists in the blood that contributes to a disease process or its symptoms

The substance can be more effectively removed by apheresis than by the body’s own homeostatic mechanisms

Category I

Indication Categories for Therapeutic Apheresis

Apheresis is standard and acceptable, either as primary therapy or as a first-line adjunct to other initial therapies

Category II

Indication Categories for Therapeutic Apheresis

Apheresis is generally accepted in a supportive role or as second-line therapy, rather than first-line therapy

Category III

Indication Categories for Therapeutic Apheresis

Apheresis is not clearly indicated based on insufficient evidence, conflicting results, or inability to document a favorable risk-to-benefit ratio

Category IV

Indication Categories for Therapeutic Apheresis

Apheresis has been demonstrated to lack efficacy or be harmful and should be discouraged in these disorders

ABC Codabar

ISBT 128

FDA recognizes two acceptable languages for blood component labeling:

Donation and Collection Facility Identifiers

ISBT 128

Upper left quadrant

Blood Type

ISBT 128

Upper right quadrant

Product Description

ISBT 128

Lower left quadrant

Expiration Date and Special Labels

ISBT 128

Lower right quadrant

100

ISBT 128 donation number is unique on a worldwide basis and not repeatable for _ years

Yellow

Blood Label Color: Type A

Pink

Blood Label Color: Type B

White

Blood Label Color: Type AB

Blue

Blood Label Color: Type O

Tan

Special Message Labels: Hold for further processing

Orange

Special Message Labels: For emergency use only

Green

Special Message Labels: For autologous use only

Gray

Special Message Labels: Not for transfusion

Purple

Special Message Labels: Irradiated

Red

Special Message Labels: Biohazard

Chartreuse

Special Message Labels: From therapeutic phlebotomy

10

Retention Time of Donor Records: All donor records are retained for ? years

5 years

Retention Time of Donor Records: Medical doctor approval for donation interval

10 years/indefinite

Retention Time of Donor Records: Notification of abnormal results

ABO/Rh Typing

Antibody Screen

Screening test for infectious diseases

Testing of Donor Blood (3)

cold, 5

Treponema pallidum is killed in _ temperature and the record should be kept for _ years

negative

Serologic Testing

If the screening test is NON-REACTIVE, the test is _

Confirmatory test

Serologic Testing

If REACTIVE, the test is repeated in duplicate. Requires _ _

Research Institute for Tropical Medicine (RITM)

confirmatory test will be done in the ?

Syphilis

Hepatitis

HIV

West Nile Virus

Chagas Disease

Different Diseases Screened Prior to the Release of Blood Products (5)

Syphilis

Hepatitis B Surface Antigen (HBsAg)

Hepatitis B Core Antibody (anti-HBc)

Hepatitis C virus Antibody (anti-HCV)

Human Immunodeficiency Virus Antibodies (anti-HIV 1/2)

Human T-cell lymphotropic virus antibody (anti-HTLV I/II)

Human Immunodeficiency Virus (HIV-1)

Hepatitis C Virus

West Nile Virus

Trypanosoma cruzi antibody

Screening Test for Infectious Diseases (10)

72, 1-6

Spirochetes in syphilis can’t survive more than _ hours inside the citrated blood stored at _-_ C