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starch: orange to black
place a small sample of the test solution on a spotting tile and add a drop of iodine solution
protein: pale blue to pale purple
add biuret reagent to the test solution in a test tube or on a spotting tile
lipid: a milky emulsion forms
place test solution in a test tube, add ethanol, then water and shake
sugar: transparent to opaque; blue to green to orange to red
place test solution in a test tube, add benedict’s reagent and heat by standing the test tube in a beaker of hot water (80 degrees celsius)