Recombinant DNA

To identify cells that have taken up the recombinant DNA

What is the purpose of a selectable marker in recombinant DNA technology?

d

Which of the following techniques is used to introduce recombinant DNA into cells?

a. Transfection

b. Termination

c. Transduction

d. Transformation

b

Which of the following enzymes is commonly used to cut DNA at specific sites in recombinant DNA technology?

a. DNA polymerase

b. Restriction endonuclease

c. Ligase

d. Reverse transcriptase

a

Which of the following is an application of recombinant DNA technology in agriculture?

a. All of these

b. Developing crops that can grow in harsh environments

c. Producing crops with enhanced nutritional value

d. Creating pest-resistant crops

Using restriction enzymes to cut the vector at a specific site and then ligating the gene of interest into the cut site

How is the gene of interest spliced into a vector?

a

In what way/s is recombinant DNA technology useful?

a. all of these

b. to produce recombinant proteins for vaccines

c. to improve crop yields and increase resistance to pests and diseases

d. to produce enzymes for food processing

c

Which of the following is an application of recombinant DNA technology in environmental cleanup?

a. Producing enzymes that can break down toxic pollutants

b. Creating bacteria that can degrade oil spills

c. All of these

d. Developing plants that can absorb heavy metals from contaminated soil

An organism that has been genetically modified with DNA from another species

What is a transgenic organism?

A technique to splice together DNA from different sources

What is recombinant DNA technology?

b

Which of the following is a type of vector commonly used in recombinant DNA technology?

a. Restriction enzyme

b. Plasmid

c. Polymerase

d. Ligase

Enzyme

proteins that catalyze biochemical reactions, and in recombinant

DNA technology, they are used to cleave DNA at specific sites.

Splice/Splicing

the process of joining two DNA fragments together

DNA

the genetic material found in living organisms

Isolate/Isolation

refers to the process of extracting a particular substance from

a mixture

Vector

a DNA molecule that is used to transfer a foreign DNA fragment into a host cell,

where it can be replicated and expressed.

True

Recombinant DNA technology involves using enzymes and various laboratory

techniques to manipulate and isolate DNA segments of interest.

recombinant DNA.

Recombinant DNA is used to combine (or splice) DNA from different species or to create genes with new functions. The resulting copies are often referred to as ___

Enzymatic cleavage

What is applied to obtain different DNA fragments?

1973

first recombinant DNA (rDNA) molecules were generated in ____ by Paul Berg, Herbert

Boyer, Annie Chang, and Stanley Cohen of Stanford University and University of

California San Francisco.

The insertion of recombinant DNA so that the foreign DNA will

replicate naturally.

Describe this diagram

involves the manipulation and isolation of DNA segments using enzymes and laboratory

techniques. This allows for the combination of DNA from different species or the

creation of genes with new functions. The resulting copies are called recombinant DNA.

Principle of recombinant DNA technology

DNA ligase

Which enzyme is used to ligate the vector and isolated gene?

Gel electrophoresis

What technique is used to separate the desired DNA fragment after enzymatic cleavage?

To identify cells with the desired phenotype

What is the purpose of a marker gene in the selection and screening of transformed cells?

Restriction

__________ enzymes are used to cleave DNA at specific sites during isolation.

Inducible promoters

__________ is used to amplify the desired gene product after introduction into the host cell.

To produce therapeutic proteins

What is the significance of recombinant DNA technology in medicine?

insulin

hormone used to regulate blood sugar levels in people with diabetes, can now be

produced using recombinant DNA technology. This has increased its availability and

reduced its cost, making it more accessible to people who need it.

to improve the nutritional content, flavor, or appearance of food, and produce additives

How is recombinant DNA technology used in food technology?

to improve crop yields, increase resistance to pests and diseases, and enhance nutritional content. For example, genes can be introduced into crops to make them more resistant to herbicides or to produce insecticidal proteins that protect the plant from pests.

How is recombinant DNA technology used in Agriculture?

increase

In the sample data, the application of K31 to the chemically damaged hairs resulted in ____ of mechanical strength.

It increases mechanical strength, diameter, tensile strength, and surface smoothness, and straightens curly hair.

In the sample data, describe the effect of keratin K31 on chemically damaged hair.