BI 203 Lab Quiz 2

Bacterial Basics

How many words make up a binomial nomenclature, and what are they called?

2 ; Genus and species

What are the names origin, and how did they come to be?

Greek or Latin, either describes some characteristic or is associated with its discoverer.

What are the rules for binomial nomenclature?

1. Genus capitalized, species lower case

2. must be italicized when typed

3. must underline both genus and species separately if written

What are the conversion for centi, milli, micro, and nanometers?

Centi ( c ) = 10 ^-2

milli (m) = 10^-3

Micro (u) = 10^-6

Nano (n) = 10 ^-9

What are the most used measurements in lab?

microliters and micrometers

What is the strength of an objective lens on a microscope?

10x

What are the four kinds of objective lens on a microscope?

1. scanning

2. low power

3. high power

4. oil immersion

What is the strength of a scanning objective lens?

4x

What is the strength of a low power objective lens?

10x

What is the strength of a high power objective lens?

40x

What is the strength of a oil immersion objective lens?

100x

How do you calculate the total zoom of a microscope?

ocular lens x objective lens = total zoom

What does EPU stand for?

Eye piece unit?

What are the four types of EPU’s?

1. Scanning

2. Low power

3. High power

4. Oil Immersion

What is the conversion for a scanning EPU?

1 EPU = 25 um

What is the conversion for a low power EPU?

1 EPU = 10 um

What is the conversion for a high power EPU?

1 EPU = 2.5 um

What is the conversion for a oil immersion EPU?

1 EPU = 1 um

What shape of bacteria is this called?

Coccus

What shape of bacteria is this called?

Coccobacillus

What shape of bacteria is this called?

Vibrio

What shape of bacteria is this called?

Bacillus

What shape of bacteria is this called?

Spirillum

What shape of bacteria is this called?

Spirochete

What kind of cells can you see with a microscope?

Bacteria, prokaryotes, Eukaryotes

About how long and wide is a bacteria?

Width: 0.4- 1.5 um

Length: 1 - 10 um

What is something you cant see with a regular microscope? What kind of microscope would you need to see it?

Viruses ; You would need an electron microscope to see these.

How big are viruses usually?

10 - 300 nm

What color is a gram positive bacteria?

Violet

What color is a gram negative bacteria?

pink

What are 7 thing you need to do to put the microscope away safely?

1. Clean lenses with lens cleaner and wipes

2. Leave the lens at the lowest objective

3. Turn down light all the way

4. Lower stage all the way

5. Neatly coil cord into cubby

6. Turn power off

7. Face microscopes with eye piece toward the back of cabinet