Fastidious Gram Negative Bacilli (Rods)

Fastidious GNR have what characteristic shape when Gram stained?

coccibacili

What is the X growth factor?

hemin

What is the V growth factor?

yeast (NAD)

What media is used to culture fastidious GNR?

CHOC

What 2 types of media are used to culture Haemophilus species?

1. CHOC

2. QUAD plate

The fourth quadrant on a QUAD plate contains _______ blood.

horse

What species of Haemophilus requires both X and V growth factors and is gamma hemolytic?

Haemophilus influenzae

What fastidious GNR exhibits satellitism with Staphylococcus aureus?

Haemophilus influenzae

What fastidious GNR can not grow in the X quadrant and V quadrant, but can grow in the XV quadrant and is gamma hemolytic in the horse blood quadrant?

Haemophilus influenza

What strain of Haemophilus influenzae causes the most serious infections of meningitis?

encapsulated serotype b

What fastidious GNR is the leading cause of meningitis in children ages 5 months to 5 years?

Heamophilus influenzae

A negative porphyrin test indicates that an organism requires ____ growth factor.

X

What species of Haemophilus requires only the V growth factor and is gamma hemolytic?

Haemophilus parainfluenzae

What Haemophilus species is the most common species isolated?

Haemonphilus parainfluenzae

What fastidious GNR can not grow in the X quadrant, but can grow in the V and XV quadrants and is gamma hemolytic in the horse blood quadrant?

Haemophilus parainfluenzae

What strain of Haemophilus influenzae causes pink eye?

Haemophilus influenzae aegypticus

What fastidious GNR causes chancroid and clusters like a “school of fish” when Gram stained?

Haemophilus ducreyi

AACEK organisms are associated with what condition when isolated from the blood?

endocarditis

What fastidious GNR has a musty/mushroom odor and can cause infections through cat bites?

Pasturella multocida

What fastidious GNR is a level 3 biohazard and causes undulant fever?

Brucella abortus

Bone marrow aspirate is one type of specimen collected to culture what fastidious GNR?

Brucella abortus

What fastidious GNR is a level 3 biohazard and causes rabbit fever?

Francisella tularensis

What fastidious GNR is cultured on blood cysteine glucose agar and incubated for 3 weeks?

Francisella tularensis

Lymph node biopsy and ulcer scrapings are types of samples collected to culture what fastidious GNR?

Francisella tularensis

What fastidious GNR causes whooping cough?

Bordetella pertussis

What is the ideal specimen for culturing Bordetella pertussis/

N/P (Dacron) swabs

Bordet-Gengou agar and Regan-Lowe agar are used to culotte what fastidious GNR?

Bordetella pertussis

What fastidious GNR causes Pontiac Fever?

Legionella pneumophila

Buffered charcoal yeast extract agar (BCYE) is used to culture what 3 fastidious GNR?

1. Brucella abortus

2. Francisella tularemia

3. Legionella pneumophila

Transtracheal aspirate (TTA) and bronchial washing (BAL) are types of specimens collected to culture what fastidious GNR?

Legionella pneumophila

What fastidious GNR has “clue cells” and causes bacterial vaginosis infections?

Gardnerella vaginalis

Human blood tween (HBT) and the whiff test are used when dealing with what fastidious GNR?

Gardnerella vaginalis

What is the colony morphology of Gardnerella vaginalis on HBT?

tiny, beta hemolytic