Bio 122 Biotechnology

gel electrophoresis

The separation of nucleic acids or proteins, on the basis of their size and electrical charge, by measuring their rate of movement through an electrical field in a gel.

Electrophoresis for DNA

Sorts into bands, longer strands end up closer to top, shorter closer to bottom

restriction enzymes

Enzyme that cuts DNA at a specific sequence of nucleotides
Mix enzyme w DNA and put on heat for sev hours
Spreads out on gel and compare
Stain

Blotting

The transfer of DNA or proteins from an electrophoresis gel to a nitrocellulose filter.

Hybridization with a probe

• The method used to detect specific nucleotide sequence in an unknown sample by using a gene probe
• Gene probe is a short segments of DNA of a known sequence
• A probe carries a radioactive label

Autoradiography

technique that relies on the emission of radioactive particles from within the subject to produce an image.
Photographic film laid over paper
Probe exposes film to image