Unit 6: Antibody Identification

Each panel will consist of type ___ reagents cells with known antibodies.

O

A (+,0) indicates a (single/double) dose.

double (homozygous is stronger reacting)

A (+,+) indicates a (single/double) dose.

single (heterozygous causes expression to be weaker)

What makes up an autocontrol for antibody identification?

patient RBCs + patient serum/plasma

We use a _______% dilution for the autocontrol when tube testing and a _______% dilution when gel testing.

2-5%

4%

How do you make the 4% dilution for gel testing?

1000 ul or 1L of 2+ + 10 ul of patient RBCs

A positive autocontrol indicate an:

autoantibody

What drug, used to treat multiple myeloma, can cause all cells to be reactive?

Darzalex

What test results for cells are used to rule out antibodies in antibody panels?

negative at all phases

We use (homozygous/heterozygous) (negative/positive) cells to rule out antibodies.

homozygous positive

What 3 antibodies can you rule out heterozygously?

1. anti-K

2. anti-Lu^a

3. anti-C and anti-E (ONLY if anti-D is present which is very rare)

What is the 3 + 3 rule?

3 positive reactions for positive result

3 negative reactions for negative result

(don’t forget SC1 and SC2 can count as one of the 3)

Positive controls for anti-sera must be (homozygous/heterozygous).

heterozygous (+ +) and (- -)

Negative controls for anti-sera must be (homozygous/heterozygous).

homozygous (- +)

If you have anti-____, you are then more likely to form other antibodies like anti-c.

anti-E

When some or all of the panel cells are positive with similar reactivity, this is indicative of an (autoantibody/alloantibody).

alloantibody

When there are varying strengths of reactions at AHG with a negative autocontrol, this is indicative of an (autoantibody/alloantibody).

alloantibody

When all of the panels cells including the auto control are positive, this indicates an (autoantibody/alloantibody).

autoantibody

A 1+ reaction for AHG phase and autocontrol of antibody panel and AHG, IgG, and elution of DAT is seen, this indicates a (warm/cold) autoantibody.

warm

What do you perform to see if there is an alloantibody being covered by an autoantibody?

adsorption

(Warm/Cold) autoantibodies can cause hemolytic anemias.

warm

What cell do we use for the autocontrol when performing a cold autoantibody screen?

cord cell (O+)

A reaction of 2+ at 4C indicates a (warm/cold) autoantibody.

cold

All negative cells during a prewarm technique confirms a (warm/cold) autoantibody.

cold

Anti-_____ is known for being refractile when observed with a microscope.

anti-Bg (Bennett-Goodspeed)