Micro 150 Ch 3.2 HW + Quiz WIP

What lens is this?

Ocular

What connects the ocular lens to the microscope?

Body

Component that holds the objective lens?

Nosepiece

Type set of 3 lens used when the microscope is on viewed through the Ocular Lens

Objective lens

“Platform” used to hold glass slide for viewing

Mechanical Stage

Component that focuses light into Mechanical stage

Aperture diaphragm control

Component that is keeps the microscope level and flat on counter surface?

Base

Component that emits the light of the microscope

Field diaphragm lever

Component that holds the Ocular, Objective, Nosepiece, and or majority of the microscope’s staging components

Arm

Greater knob paired with a finer knob. Used in x4 an or x10

Coarse focus

A “Fine” knob used to focus and or adjust slide image at a higher magnification

Fine focus

Component used to adjust stage

Stage adjustment

Good magnified image consists of what concepts?

All of these options

Resolution is influenced by?

Wavelength & Numerical Aperture

Contrast is increased by

Staining

Type of stain that Stains the specimen

Positive Stain

Type of Stain that stains the Background

Negative Stain

Stain method of Negative Stain

Capsule Stain

Stain method(s) of Positive Stain

All of the three options

Differential Stain associates with what other Stain method

Gram Stain - Determines presence of LPS Layer

Acid Fast Stain - ID mycobacterium tuberculosis

Stain method Associated with Special Stain

Flagellar Stain - Defines means of motility

Differential Stains

Distinguishes organisms

The Gram Stain - permits the viewer to distinguish between gram-positive and gram-negative bacteria

Simple Stains

Easy ID of Shape and Arrangement

The methylene blue stain - Uses one dye

Special Stains

Illustrate certain structures

The flagellar stain

Magnification

The apparent increase in size of an image

Contrast

The ability to be distinguished from the surroundings

Resolution

The ability to distinguish between two separate structures that are very close to one another

Bacteria are larger than human cells. T/F

False

Bacteria are only visible with an electron microscope. T/F

False

The term used to describe a cluster of spherical bacteria is _______.

staphylococci

What general type of stain is used to separate types of bacteria based on their cellular structures?

Differential

Why must fresh cells be used when performing a Gram stain?

Old cells may not Gram stain properly Correct

After Gram's iodine is added, what color do the cells appear under a light microscope?

All cells appear purple

When ethanol is applied correctly, gram-positive cells appear ______ and gram-negative cells appear ______.

purple; colorless

The thinner peptidoglycan layer of gram-positive bacteria allows the crystal-violet-iodine complex to leave the cell. T/F

False

The differential stage of the Gram stain is the application of _______.

Ethanol

If the Gram's iodine step was skipped in Gram stain procedure, what color would the cells likely be when viewed under the microscope?

Most cells would appear red/pink

All bacteria can be classified as either gram-positive or gram-negative. T/F

False

The presence of flagella can be determined by a Gram stain. T/F

False

Rod-shaped bacteria _______.

can be either gram-positive or gram-negative