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Vocabulary-style flashcards covering key terms and definitions from the notes on prokaryotic and eukaryotic cell structure and function.
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coccus (cocci)
Spherical-shaped bacterium.
bacillus (bacilli)
Rod-shaped bacterium; plural bacilli.
Bacillus (genus)
A bacterial genus; Bacillus anthracis is the causative agent of anthrax.
bacillus (shape)
Rod-shaped bacterial morphology (not necessarily in reference to the genus).
vibrio
Bacteria that look like curved rods.
spirillum
Spiral-shaped, rigid bacterium with a helical corkscrew shape.
spirochete
Spiral, flexible bacteria moved by axial filaments; typically corkscrew-like and capable of twisting.
axial filament
Endoflagellum; a corkscrew-like filament inside a sheath that enables spirochete movement.
glycocalyx
A sugary coat surrounding a cell; can be organized as a capsule or unorganized as a slime layer.
capsule
A well-organized, tightly attached glycocalyx layer surrounding some bacteria.
slime layer
An unorganized, loosely attached glycocalyx coating.
EPS (extracellular polymeric substance)
Polysaccharide/protein matrix that helps biofilm formation and adherence.
biofilm
Community of cells embedded in a glycocalyx EPS on surfaces.
capsule and virulence
Capsules can protect bacteria from phagocytosis, contributing to virulence.
polyglutamic acid capsule
Capsule of Bacillus anthracis made of p-glutamic acid; protects against phagocytosis.
polysaccharide capsule
Capsule made of polysaccharides (e.g., Streptococcus pneumoniae) contributing to virulence.
phagocytosis
Ingestion and digestion of microorganisms and solids by host cells.
Streptococcus pneumoniae capsule
Polysaccharide capsule enabling pneumonia-causing virulence.
Klebsiella capsule
Capsule that prevents phagocytosis and aids adherence in the respiratory tract.
Streptococcus mutans
Oral bacterium that uses its glycocalyx/capsule to attach to teeth and may utilize capsule as energy source.
Vibrio cholerae glycocalyx
Glycocalyx aiding attachment to intestinal cells.
flagellum
Long, whip-like appendage used for bacterial motility.
axial filament (endoflagellum)
Motility mechanism in spirochetes; flagella inside an outer sheath enabling corkscrew motion.
peritrichous
Flagella distributed around the entire cell.
polar flagellum
Flagellum(s) at one or both ends of the cell.
monotrichous
A single flagellum at one pole.
lophotrichous
Tuft of flagella from one pole.
amphitrichous
Flagella at both poles.
H antigen
Flagellar protein used to distinguish serovars among gram-negative bacteria.
archaeella (archaellum)
Motile structure in Archaea similar to flagella, but distinct evolutionarily.
spirochete movement
Movement by axial filaments producing a corkscrew motion.
fimbriae
Hairlike appendages aiding adhesion to surfaces and other cells; numerous per cell.
Pili (pilus)
Longer than fimbriae; involved in motility (twitching) and DNA transfer (conjugation).
twitching motility
Jerky movement via pilus extension and retraction.
gliding motility
Smooth movement across surfaces, seen in some bacteria like myxobacteria.
conjugation (sex) pilus
Pilus used to transfer DNA between bacteria during conjugation.
gram-positive cell wall
Thick peptidoglycan layer; teichoic and lipoteichoic acids present.
gram-negative cell wall
Thin peptidoglycan layer plus outer membrane containing LPS; periplasmic space exists.
peptidoglycan
Disaccharide NAM-NAG polymer cross-linked by peptides; provides cell wall rigidity.
NAM/NAG
N-acetylmuramic acid and N-acetylglucosamine; backbone units of peptidoglycan.
teichoic acid
Negatively charged polymers in gram-positive cell walls; contribute to wall charge and antigenicity.
lipoteichoic acid
Teichoic acid extending to the plasma membrane; helps anchor wall components.
lipopolysaccharide (LPS)
Outer membrane component in Gram-negative bacteria with lipid A, core polysaccharide, and O polysaccharide.
lipid A
Endotoxin part of LPS; triggers fever and inflammatory responses.
O polysaccharide
O antigen of LPS; serves as serovar/antigen marker in gram-negative bacteria.
porins
Proteins forming channels in the outer membrane to allow molecule passage.
Gram stain
Differential stain; gram-positive retain crystal violet; gram-negative decolorize and take safranin.
Mycoplasma
Bacteria lacking cell walls; membranes contain sterols; resistant to typical cell-wall-targeting antibiotics.
pseudomurein
Cell wall component in some Archaea similar to peptidoglycan but with different sugar acids.
acid-fast cell wall
Mycobacterial cell wall rich in mycolic acids; stained with carbolfuchsin; resistant to Gram stain.
lysozyme
Enzyme that hydrolyzes peptidoglycan bonds; damages bacterial cell walls.
EDTA
Stabilizes outer membrane disruption to allow lysozyme access in Gram-negative bacteria.
protoplast
A wall-less cell with plasma membrane intact after cell wall removal.
spheroplast
Gram-negative cell after cell wall removal leaving outer membrane intact.
mesosome
Membrane folds in bacteria; now considered an artifact from electron microscopy.
fluid mosaic model
Phospholipid bilayer with embedded proteins; membrane components move laterally.
peripheral vs integral proteins
Peripheral proteins lie on membrane surfaces; integral proteins span the membrane.
selective permeability
Membrane allows some substances to cross while restricting others.
diffusion
Net movement of small, nonpolar molecules from high to low concentration.
facilitated diffusion
Diffusion via membrane transport proteins; no energy expenditure; specificity varies.
osmosis
Net movement of water across a selectively permeable membrane.
isotonic/hypotonic/hypertonic
Isotonic: equal solute concentration; hypotonic: lower outside; hypertonic: higher outside.
active transport
Energy-dependent transport against a concentration gradient.
group translocation
Prokaryote-specific active transport where the transported substance is chemically modified during transit (e.g., glucose phosphorylation by PEP).
endocytosis
Eukaryotic process of taking in material by vesicle formation; includes phagocytosis, pinocytosis, and receptor-mediated endocytosis.
cytoplasm
Cell contents within the plasma membrane, about 80% water; contains cytosol, organelles, and inclusions.
cytoskeleton (prokaryotes)
MreB, ParM, cresetin, and FtsZ proteins providing shape, division, and organization.
nucleoid
Region in prokaryotes containing the circular bacterial chromosome; no membrane around DNA.
plasmids
Small, circular, extrachromosomal DNA molecules that replicate independently and often carry advantageous genes.
70S ribosomes
Prokaryotic ribosomes (30S + 50S subunits) smaller than eukaryotic 80S ribosomes.
80S ribosomes
Eukaryotic ribosomes (60S + 40S subunits) larger than bacterial 70S ribosomes.
carboxysome
Inclusion containing RuBP carboxylase used in CO2 fixation in photosynthetic bacteria.
gas vacuoles
Protein-lined hollow cavities that provide buoyancy in aquatic prokaryotes.
magnetosome
Iron oxide inclusions that act like magnets to orient bacteria and may protect against reactive oxygen species.
endospore
Durable, dormant, highly resistant resting cell formed by some Gram-positive bacteria under nutrient depletion.
sporulation
Formation of endospores inside vegetative cells.
dipicolinic acid (DPA)
Molecule in endospore core aiding dehydration and resistance.
germination
Process by which an endospore returns to vegetative cell under favorable conditions.
nucleus
Membrane-bound organelle in eukaryotes containing most DNA.
nuclear envelope
Double membrane surrounding the nucleus with nuclear pores.
nucleolus
Region where ribosomal RNA (rRNA) is synthesized.
endoplasmic reticulum (ER)
Network of membranes; rough ER has ribosomes; smooth ER lacks ribosomes and functions in lipid synthesis and detoxification.
Golgi complex
Organelle that processes, sorts, and ships proteins via vesicles; cisternae stack.
lysosome
Membrane-bound vesicle with digestive enzymes; digests ingested material.
vacuole
Membrane-bound space used for storage and endocytosis in cells; plant vacuoles can occupy much of the cell.
mitochondrion
Organelle producing ATP via cellular respiration; contains cristae and matrix; has 70S ribosomes and its own DNA.
cristae
Folds of the inner mitochondrial membrane increasing surface area for respiration.
matrix
Central fluid within mitochondria where many enzymes reside.
chloroplast
Plant/algal organelle for photosynthesis; contains chlorophyll, thylakoids, grana, and 70S ribosomes.
thylakoid
Flattened membrane sacs in chloroplasts where light reactions occur.
grana
Stacks of thylakoids in chloroplasts.
peroxisome
Organelle with enzymes for oxidation reactions and detoxification; contains catalase to break down hydrogen peroxide.
centrosome
Cellular region near the nucleus organizing the spindle during mitosis; contains centrioles and pericentriolar material.
centriole
Nine triplet microtubules arranged in a 9+0 array within the centrosome.
endosymbiotic theory
Hypothesis that eukaryotic organelles evolved from free-living prokaryotes living inside larger host cells.
eukaryotic endocytosis
Process of taking in substances by invagination of the plasma membrane; includes phagocytosis, pinocytosis, and receptor-mediated endocytosis.
monomorphic
most bacteria are this, that is, they maintain a single shape
pleomorphic
bacteria that can have multiple shapes