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Rendered Animal Proteins and Fats

U.S. Animal Agriculture Annual Production – 2017
Livestock Production
• 32 million cattle → 49% of live weight not used for human food
• 121 million hogs → 44% not used for human food
• 9 billion chickens → 37% not used for human food
• 242 million turkeys → 36% not used for human food
Meat Production
• U.S. meat production → 24 billion kg
• Approximately 10 billion kg → meat by-products
Poultry Production
• U.S. poultry production → 22 billion kg
• Approximately 8 billion kg → poultry by-products

Eating High Off the Hog
Meaning
• “Eating high off the hog” → refers to eating what many consider choice cuts of meat
• These cuts are found high on the body of an animal
• Examples → shoulders, hams, steaks, roasts, and chops
Changes in Eating Habits
• In the past, it was said that many Americans ate every part of the pig except the squeal
• Over the last few decades, many Americans have become more particular about what parts of animals they eat
• Parts that are nutritious and were previously considered delicious are now widely avoided
Co-Products / By-Products
• In many places around the world, these animal parts are still considered delicacies
• In the United States, nutritious animal parts that people choose not to eat are often called byproducts
• In reality, they are co-products of the steaks, chicken breasts, and hams that are preferred

Agrarian Society
Butchering and Food Use
• In an agrarian society → society based largely on agriculture and farming
• After butchering, many parts of the animal were used and preserved rather than discarded
• Examples → sausages, headcheese, lard, white salt-pork, smoked hams, and shoulders
• These foods show how different parts of the animal were used for food

Gastronomical Treasures
Animal Co-Products as Foods
• Fromage de tête (Head cheese) → Germany / delicatessen
• Tête fromagée (Head cheese) → France / Canada
• Salade de gésiers (Gizzard salad) → France
• Tripes à la mode de Caen (Caen-style tripes) → France
• Thalia ou Douara (Lamb offals stew) → Oum Rayane
• Chicken feet dim sum → China

Centralization of Meat Packing Facilities
Large-Scale Facilities
• 500 animal pens covered 60 acres of land
• Facility could accommodate at one time:
• 21,000 cattle
• 75,000 hogs
• 22,000 sheep
• 200 horses

Interrelationships of Rendering with Animal Agriculture
Animal Production
• Animal production → animals are raised for agricultural purposes
• Animal slaughter → produces by-products
• On-farm deaths → also provide material for rendering
Rendering
• Rendering → processes animal by-products
• Materials include fat, bone, and trim
Uses of Rendered Products
• Feed uses → rendered products can be used in animal feed
• Non-feed uses → rendered products can be used for other purposes
• Meat processing → connected with the production and use of animal by-products
• Retail meat → meat produced for consumers
Importance of Rendering
• Rendering plays an essential role in an environmentally, ethically, and economically sustainable agriculture industry

Value Chain for Animal Products / Co-Products
Uses
1. Human Food
2. Pharmaceutical and Fine Chemistry
3. Pet Food
4. Animal (Livestock) Feeds
5. Industrial Uses
6. Fertilizers
7. Fuels
Rendering
• Rendering is the central process connecting animal products/co-products to these different uses

The Rendering Industry – U.S. and Canada
Industry Size
• 170 facilities in the U.S.
• 10 facilities in Canada
• $10 billion annual revenue
Raw Material
• 27.5 MMT (62 billion lb) of raw material processed each year
Meat and Bone Meal (MBM)
• Approximately 2.5 million metric tons of meat and bone meals (MBMs) produced
• MBMs can be of bovine, porcine, or mixed species origin
• Bovine → cattle
• Porcine → pigs
Exports
• A significant proportion of North American MBM production is exported
• Exported to many different regions of the world

Diversity of Raw Materials
Raw Materials Used in Rendering
• Offal → internal organs and other parts not typically used as meat
• Bones and fat
• Blood
• Animals dead on arrival, in transit, or on farms
• Restaurant grease
• Feathers
• Recalled meat

Raw Materials
By-Products
• Hides, skins, hair, feathers
• Hoofs, horns, feet, heads
• Bones, toe nails
• Blood, organs, glands, intestines
• Muscle and fat tissues
• Shells
• Whole carcasses
Composition Before Rendering
• Raw materials vary, but an overall approximation is:
• 60% water
• 20% protein and mineral
• 20% fat
Perishability and Microorganisms
• These organic materials are highly perishable
• Laden with microorganisms → many can be pathogenic to humans and animals
Importance of Rendering
• Rendering provides a safe and integrated system for animal raw material handling and processing
• Complies with fundamental requirements for:
• Environmental quality
• Disease control

Specific Risk Materials (SRMs)
Definition
• In both the United States and Canada, SRMs (Specified Risk Materials) are defined as parts from cattle aged 30 months or older
• SRMs include:
• Skull
• Brain
• Trigeminal ganglia → nerves attached to the brain and close to the skull exterior
• Eyes
• Spinal cord
• Distal ileum → part of the small intestine
• Dorsal root ganglia → nerves attached to the spinal cord and close to the vertebral column
Feeding Restrictions
• In Canada, enhanced animal health safeguards were implemented in July 2007
• It is illegal for SRMs to be fed to any animal, including dogs and cats
• The United States has similar rules

The Basic Production Process of Rendering
Rendering Process
• Raw Materials → animal by-products enter the rendering process
• Sizing → raw materials are reduced to an appropriate size
• Heat Processing → materials are heated using controlled time × temperature
• Protein Press → separates protein material from fat
• Grinding → processed protein material is ground
• Fat Clean-up → fat is cleaned/purified
• Storage/Load out → finished products are stored and prepared for shipment
Process Control / GMP Points
• Process control and GMP (Good Manufacturing Practices) points are used throughout the rendering process
• Help ensure the process is properly controlled and products are handled safely

Rendering is Cooking and Drying
Rendering Process
• Rendering involves cooking and drying animal materials
• Can be done as:
• Continuous flow → materials move continuously through the process
• Batch → materials are processed in separate batches
• Uses steam cookers
• Processing temperature → 115–145°C (245–290°F)
• Processing time → 40–90 minutes
Purpose of Rendering
• Provides a sanitary and eco-friendly way to dispose of the massive amount of meat and food by-products produced every year
• These materials spoil easily and provide an excellent medium for pathogens to grow and multiply
Disease Control
• Temperatures used during processing kill conventional disease-causing organisms
• Examples → bacteria and viruses

Examples of a Few Finished Products
Finished Products
• Stabilized poultry fat
• Hydrolyzed poultry feather meal
• Stabilized pet food poultry fat
• Stabilized poultry protein meal
• Low ash pet food poultry protein meal
• Pet food grade poultry protein meal

Efficacy of the U.S. Rendering System in the Destruction of Pathogenic Bacteria.

Protein Meals
Production
• 5 million metric tonnes of protein meals
• 125 AAFCO-defined animal by-products
Types of Protein Meals
• Meat meal
• Meat and bone meal → can be species specific
• Blood meal → flash, spray, ring, or batch dried
• Poultry by-product meal
• Poultry meal
• Hydrolyzed poultry feather meal

U.S. Production of Rendered Fats

Fatty Acid Composition of Common Feed-Grade Oils and Fats

Examples of AAFCO Definitions
Meat and Bone Meal
• Rendered product from mammal tissues, including bone
• Excludes:
• Blood
• Hair
• Hoof
• Horn
• Hide trimmings
• Manure
• Stomach and rumen contents
• These materials are excluded except for amounts that unavoidably occur during good processing practices
Poultry By-Product Meal
• Ground, rendered, clean parts of the carcass of slaughtered poultry
• Examples → necks, feet, undeveloped eggs, intestines
• Excludes feathers, except for amounts that unavoidably occur during good processing practices
Poultry Fat (Feed Grade)
• Primarily obtained from poultry tissue during commercial rendering or extraction
• Contains only the fatty matter natural to the product produced under good manufacturing practices
• Contains no added free fatty acids or other materials obtained from fat
• Must contain:
• ≥ 90% total fatty acids
• ≤ 3% unsaponifiables and impurities
• Minimum titer of 33°C → temperature-related measure of the fat
• If an antioxidant is used → common name(s) must be listed, followed by “preservative(s)”
AAFCO
• Association of American Feed Control Officials
• A voluntary membership association of local, state, and federal agencies
• Members are charged by their local, state, or federal laws to regulate the sale and distribution of animal feeds and animal drug remedies in the USA

Commodity Prices of Several Rendered Protein Meals

Insights from 30 Years of Research and Cooperation with Aquafeed Industry
Rendered Animal Protein Ingredients
• Rendered animal protein ingredients are among the most cost-effective feed ingredients for aquaculture feeds
• Least-cost feed formulation programs often seek to include 20–35% of these ingredients in feeds for a variety of species
Industry Concerns
• Aquaculture feed manufacturers are reluctant to use high levels → e.g. more than 10–15%
• Reasons → quality variability and uncertainty about the nutritive value of these ingredients
Nutritive Value Research
• Better characterization of nutritive value is very important to the feed industry
• Especially important → digestibility and bio-availability of amino acids
• Digestibility → how well nutrients can be digested and used
• Bio-availability → how much of the nutrient is available for the animal to use

Least-Cost Feed Formulation for Asian Seabass
Feather Meal Inclusion
• Least-cost feed formulation for Asian seabass calling for 37% feather meal
• Unrealistic inclusion level, but demonstrates that rendered animal proteins can be:
• Nutritionally well-suited
• Competitively priced

Least-Cost Feed Formulation for Asian Seabass
Rendered Animal Protein Inclusion
• Least-cost feed formulation calls for:
• 17% meat and bone meal
• 8% blood meal
• 12% feather meal
• Total → 37% rendered animal proteins
Significance
• Still slightly unrealistic
• Shows again how nutritionally well-suited and competitively priced rendered animal proteins are

Economic Value of Nutrients and Attributes
Attributes — Index: Premium or Penalty
• Marine protein → 403–727
• Non-FIFO marine protein → −49
• Vegetable protein → −177
• Land animal protein → value shown on slide
Nutrients — Index or $/tonne
• Digestible protein → 1370 ^ *
• Crude lipids → 752
• Starch → 245
• Digestible methionine → 1610
• Digestible phosphorus → 2281
• EPA + DHA → 2102
Note
• Values for nutrients are before correction for attributes
• Premium → increases economic value
• Penalty → decreases economic value

Fermentation Products
Yeast and Derivatives
• Yeast and derivatives
• Brewer’s yeast
• Beta-glucans, mannan oligosaccharides (MOS), nucleotides, etc.
Amino Acids
• L-Lysine
• L-Threonine
• L-Tryptophan
• L-Arginine
• L-Histidine
• L-Isoleucine
• Etc
Single Cell Proteins
• Bacteria → e.g. methane-consuming bacteria, CO₂-fixing bacteria
• Algae → e.g. algae biomass rich in highly unsaturated fatty acids, such as DHA

Yeast Market
Global Yeast Market
• Global yeast market estimated at USD $3.9 billion in 2020
Key Players in Yeast Products for Animal Feeds
• Alltech → USA
• Diamond V – Cargill → USA
• Angel Yeast → China
• Lallemand Inc. → Canada
• Lesaffre Group → France
• Biorigin → Brazil
• Novonesis → Denmark
• Formerly Chr. Hansen Holdings A/S + Novozymes

Product Function: Yeast
Yeast Components
• Yeast → contains yeast cell wall and yeast extract
Yeast Extract
• Contains:
• Protein
• Amino acids
• Active peptides
• Nucleotides
• Vitamins
• Active enzymes
• Immune polysaccharides
Inner Cell Wall – β-Glucan
• β-glucan stimulates the body to produce lymphocytes
• Helps the body produce specific immunity against pathogenic bacteria
• Activates macrophages in the body
Outer Cell Wall – Mannan Oligosaccharides (MOS)
• Mannan oligosaccharides (MOS) are functional polysaccharides with many biological activities
• Can improve animal production performance
• Can adsorb mycotoxins → bind mycotoxins
• Can inhibit pathogenic bacteria
• Can enhance the immune system’s defenses

Supplemental Amino Acids
Other Names
• Also known as crystalline, synthetic, or free amino acids
Production
• Obtained through microbial fermentation
Examples
• L-Lysine
• L-Threonine
• L-Tryptophan
• DL-Methionine → synthesized chemically

Algal Biomass
Most Common Examples
• Schizochytrium sp. algae
• Corbion’s AlgaPrime DHA
• Veramaris’ algal oil

Formulation of the six experimental diets with increasing level of algal meal rich in DHA and SAFA

Apparent Digestibility Coefficient (ADC) of Lipid
Study
• Rainbow trout were fed experimental diets containing increasing, graded levels of algae meal
• Source → Bureau et al. (unpub.)
Lipid Digestibility
• Significant decrease in lipid digestibility as algae meal inclusion increased
• Decreased from close to 95% to about 83%
• Almost 0.75% decrease in lipid digestibility per 1% algae meal inclusion

Growth Performance of Rainbow Trout
Study
• Rainbow trout with initial average body weight of 35 g/fish
• Fish were fed the experimental diets for 12 weeks
• Source → Bureau et al. (unpub.)
Results
• Lower digestible lipid intake
• Due to lower digestibility of saturated fatty acids
• No effect on growth
• No effect on feed efficiency

Single Cell Proteins / Microbial Biomass
Methanotrophs
• Bacteria able to use methane → e.g. natural gas
• Example → Methylococcus capsulatus
Heterotrophs
• Microorganisms that use organic matter as a source → e.g. grains
CO₂-Fixing Bacteria
• Some bacteria are able to fix CO₂

Insect Biomass
1. Black Soldier Fly (BSF)
• Black Soldier Fly (BSF) → Hermetia illucens larvae
• Able to grow on a wide variety of substrates → e.g. wastes
2. Superworm
• Superworm → Tenebrio molitor
• Fed grains / bran
• Quite specific in food choice
3. Crickets
• Crickets

Insect Protein Market
Market Potential
• Rabobank (2021)
• Global market potential of up to 500,000 metric tons by 2030 for insect protein as an animal feed ingredient
• Current volumes of insect protein estimated at 10,000 metric tons
Cost
• Insect protein is currently about 3× more expensive than regular fish meal
• As expensive as the best-quality fish meal

The exponential increase in capital flowing to insect farming companies

Worldwide oilseed production in 2021/2022, by type

Why Produce More Oilseeds?
Main Reason
• Oilseeds are produced in very large amounts because they provide both oil and protein
• After oil is extracted, the remaining oilseed meal is a valuable high-protein animal feed ingredient
• This makes oilseeds useful for producing multiple valuable products → oil + protein

Omnivorous Fish Feed Formulations
Feed Composition
• Complex mixtures of many different ingredients
• Globally, aquafeed can now be described as “plant-based feeds”
• Plant-based feeds use complementary ingredients/additives from:
• Animal origins
• Microbial origins
Focus on New Ingredients
• A lot of focus is placed on the 10% of feed mass → e.g. insects, feed additives, SCP (single cell proteins), etc.
• Very little focus is placed on the remaining 90% of feed mass
• Question raised by the slide → Is this normal?

What’s Next?
Feed Resource Quality
• Cannot meaningfully discuss the quality of feed resources without considering their specific chemical components and their properties
• Example → digestibility
Next Steps
• Review the different chemical components of feeds
• Learn how we can measure these components
• Assess whether components are:
• Digestible → can be digested by the animal
• Bio-available → usable by the animal
• Toxic → harmful to animals
Connection to NUTR 3200
• Some of this material may overlap with NUTR 3200 Fundamentals of Nutrition
Animal Nutrition Perspective
• Material will be reviewed from an animal nutrition perspective
• Objectives, emphasis/focus, and methodological approaches are often different from human nutrition
