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Level 1
double stranded DNA (b form)
Level 2
nucleosome (structural unit of chromatin)
147bp segment wraps around core of histone proteins 1.7x
histone = basic protein found in chromatin that associates with DNA (positively charged amino acids interact with negatively charged DNA)
histone octamer = globular protein structure at centre of nucleosome made of 4 pairs of histones (H2A, H2B, H3, H4)
level 3
chromosome (structural unit of chromatin)
linker histone (H1) binds to nucleosome
level 4
chromatin = complex of DNA + histones + proteins
chromatin is dispersed throughout the nucleus in interphase cells
level 5
chromatosomes
repeat every around every 200bp
connected by linker DNA (25-75bp)
‘beads on a string’ image
level 6
histone interactions cause nucleosomes to fold in 30nm fibre (attached to protein scaffold)
level 7
interphase chromatin
30nm fibres condesnse further into 250nm fibres
specific DNA sequences in interphase chromatin are available for cellular processes (e.g. transcription, replication)
step 8
chromosomes = structural unit of genetic matieral consisting of linear double stranded DNA and associated proteins (eukaryotes)
during mitosis chromatin condesnses into visible metaphase chromosomes