Required Practicals ALL Sciences

Biology, Chemistry, Physics

BIOLOGY

10 Required Praticals

Microscopy RP Method

1. Peel off an epidermal layer on the onion using forceps

2. Mount onto the microscope slide with a drop of water using a pipette, making sure the tissue lies flat

3. Add 2 drops of iodine solution to stain the cells

4. Place the cover slip on by first placing one edge down on the slide and slowly lowering the other side of the cover slip using forceps. Make sure no air bubbles are trapped!!

5. Remove any excess stain by soaking it with paper towels

6. Place the slide on the stage of the microscope

7. Turn the nosepiece to select a low power objective

8. Set up the microscope - use the coarse adjustment knob to raise the stage until the cover slip just touches the objective

9. Look into the eyepiece and turn the course adjustment knob to move the stage away until the image comes into focus

10. Turn the nosepiece to select a high power objective

11. Repeat the same process as above and look into the eyepiece to ensure the image is at a high power objective

12. Make a labelled drawing of a few cells you can see, including any features (nucleus). Write down the magnification!

13. Repeat these steps using a prepared slide

Safety precautions: wear safety goggles when handling iodine solution

List the Aim and Equipment of the Microscopy RP.

Aim: to use a LIGHT microscope to investigate a selection of plant and animal cells.

Equipment:

• Light microscope

• Microscope slide

• Cover slip

• Onion

• Forceps

• Iodine solution

• White tile

• Scalpel

• Any other prepared plant and animal slides

Microbiology RP Method

1. Spray the bench you are working on with disinfectant

2. On the BOTTOM of the agar plate, mark with a permanent marker: 3 segments, a dot in the middle of each segment, your initials, date, and name of bacteria

3. Wash your hands with antibacterial handwash

4. Place the different antiseptics onto different filter paper discs

5. Lift the lid of the agar plate at an angle carefully at an angle carefully and use forceps

TO BE CONTINUED