Lab 2: Lab Equipment & Use

The following specimen was observed at 200x total magnification. A reticle is shown. At 100x total magnification, one subdivision of the reticle represents 10 microns

The diameter of the glass rod is: 

160 microns

The following cell preparation was co-stained with DAPI (stains nuclei blue) and a red dye that labels cilia. You properly mount the cells on a compound microscope using a high-power objective and move the specimen to the middle of the focal plane (indicated by the orange box).

 

By raising the stage slowly, 

 

blue structures will appear, and you would never see red structures

blue structures will appear, and then you would see red structures 

Red structures will appear, and then you would never see blue structures 

Red structures will appear, and then you would see blue structures

Neither red or blue structures would appear.

blue structures will appear, and you would never see red structures

You generate the following DCPIP standard curve showing absorbance at 600 nm versus concentration.  You want to determine the absorbance of a 1 in 20 diluted solution that was originally 700uM. 

 

What would be the absorbance of the diluted solution? Your answer should only include one decimal place. 

0.7

700/2 = 350

35 × 0.02 = 0.7

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