bio 300L exam 1

community structure

organization of species within an ecosystem, focusing on species diversity, abundance, and interactions that define the ecological relationships in that environment

you collected 120 invertebrates from a stream, and 50 of them were mayflies. calculate species abundance (pi)

50/120= 0.42

species richness

number of different species present in a particular community or ecosystem

species evenness

how individuals are distributed across the different species in a community, indicating how balanced the population sizes of each species are

Describe the results of today’s lab in terms of which environment had greater diversity, evenness, and richness, and describe and explain your conclusion about which is likely to be a “healthier” body of water

Prepare a relative abundance curve (species abundance curve) given the appropriate data set

Describe how species richness is calculated (qualitatively)

number of different species present in a given area or community

calculate the Shannon index given the equation for it and the set of pi values for a set of species

Micropipette Volumes
P-10 1 μL – 10 μL
P-20 2 μL – 20 μL
P-100 10 μL – 100 μL
P-200 20 μL – 200 μL
P-1000 100 μL – 1 m

convert 1.0 mL, 0.1 mL, 0.01 mL, 0.001 mL

1000 ul, 100 ul, 10 ul, 1 ul

Beer's Law

Suppose all 50 colonies grew on both Str-Leu plates (1 and 7) from Bacterial Gene Transfer II and 48 grew on the His- plate (2). What is the % inheritance of the histidine gene?

48/50= 0.96 ×1-00 = 96%

Prepare a relative abundance curve (species abundance curve) given the
appropriate data set

community ecology

study of the interactions and relationships between different species within a specific area or ecosystem

Explain what the Shannon index is and what it is used for

Describe the results of today’s lab in terms of which environment had greater
diversity, evenness, and richness, and describe and explain your conclusion about
which is likely to be a “healthier” body of water

PIE

how many microliters of a stock solution of protein that’s 36 mg/mL would you transfer to get 6 mL of a 0.08 mg/mL solution?

13.3 uL

You’re given 1.5 mL of a 9 mM stock solution of NADPH. You’re asked to make exactly 5 mL of a diluted solution with a spectrophotometer absorbance value of 1.2. The molar extinction coefficient for NAPDH is 7105 L/mol·cm and the path length is 1.3 cm. The volume of the tube is 5 mL.
How many microliters of stock NADPH do you transfer?

Abs = ℇ C X

1.2 = (7105 L/mol•cm )(C)(1.3 cm)
C = 0.0001299 M
(0.009 M) V1 = (0.0001299 M)(5 mL)
V1 = 0.072.2 mL = 72.2 μL

You need to make a solution of DCMU. You want a final concentration of 3×10^-4 M in a final volume of 4 mL, and this needs to be achieved using a single 50 microliter transfer using the micropipette.

How much DCMU should you weigh out to dissolve in 10 mL of alcohol? The molecular weight of DCMU is 400 g/mol.

C1V1 = C2V2
(C1)(0.050 mL) = (0.0003 M)(4 mL)
C1 = 0.024 M

0.024 mol/L * 400 g/1 mol * 0.010 L= 0.096 g

You want to make 50 mL of a 0.7 molar naphthol yellow solution. We know that we will need 50 mL of distilled water, but how many grams of naphthol yellow do we need to add to the water?

12.537 grams