Anatomy and Physiology- Microscope Parts

BIOL 2301

Base

supports the microscope

Arm

Along with the base, used to support the microscope when carried

Objective lenses

Have magnification ranging from 4x to 40x

Light source

Projects light upward through the diaphragm and stage

Diaphragm

Regulates the amount of light on the specimen

Ocular lens

Has a magnification of 10x

Eyepiece

Contains the ocular lens

Nosepiece

Rotates the objective lenses

Stage clips

Holds the slide in place

Slide

Where the specimen is placed

Coverslip

Protects the specimen from the objective lenses

Specimen

The object being studied

Coarse adjustment knob

Moves the stage up and down for focusing

Fine adjustment knob

Moves the stage slightly to sharpen the image

How do you calculate total magnification?

(Objective X Ocular)

Red band- Scanning

4 (objective) X 10 (ocular)= 40x

Yellow- Low

10 (objective) X 10 (ocular)= 100x

Blue- High

40 (objective) X 10 (ocular)= 400x

What are the steps to focusing a microscope?

1. Turn on switch, make sure knob underneath is clockwise (as much light)

2. Make sure condenser is as close as possible to the stage.

3. Irish diaphragm all the way to the right.

4. Putting the slide on and then put the stage all the way up.

5. Find your image using coarse adjustment, then fine adjustment knob (don't move it, if you can't find the image)

6. Switch to low power, fix the fine adjustment knob.

7. Switch to high power, fix with fine adjustment knob.