BMSC 240 Laboratory Techniques - Module 8 & 9 Review

These flashcards cover key concepts related to immunology, DNA cloning, and relevant laboratory techniques as presented in the lecture notes.

Ouchterlony Test

A test that involves clumping of proteins at defined positions in a plate, allowing visualization of protein-sized complexes.

Co-localization Studies

Imaging technique that uses multiple colors to show proteins that co-localize within a cell.

Fluorescence Resonance Energy Transfer (FRET)

A technique that measures protein-protein interactions by transferring energy between two fluorescent targets that are in close proximity.

Antiparallel

The orientation of two strands of DNA where one strand runs in a 5' to 3' direction while the other runs in a 3' to 5' direction.

Plasmid

A small, circular piece of DNA that can replicate independently of chromosomal DNA and can carry genetic information.

Restriction Enzymes

Enzymes that cut DNA at specific sequences; used in molecular cloning and analysis.

Agarose Gel Electrophoresis

A technique used to separate DNA fragments based on size using an electric field to move the negatively charged DNA through a gel matrix.

conjugative plasmids

are types of plasmids that facilitate the transfer of genetic material between bacteria through the process of conjugation, often enhancing antibiotic resistance and other traits.

resistance plasmids

Plasmids that carry genes providing bacteria with resistance against antibiotics or toxins, allowing for survival in hostile environments.

colicinogenic plasmid

A type of plasmid that encodes colicins, which are proteins toxic to other bacteria, thus giving the host bacteria a competitive advantage.

degradative plasmids

are plasmids that carry genes enabling the breakdown of complex organic compounds, allowing bacteria to utilize unique carbon sources for growth.

virulence plasmids

Plasmids that carry genes responsible for enhancing the pathogenicity of bacteria, enabling them to cause disease more effectively.