Host Basics_ Gordonia terrae

Gordonia terrae

A strain of bacterium commonly used as a host for phage isolation in SEA-PHAGES research. It is a gram-positive soil organism with the ability to breakdown various hydrocarbons, making it suitable for bioremediation and industrial biotechnology.

Actinobacteria

A phylum of bacteria to which Gordonia terrae belongs. Actinobacteria are known for their genetic diversity and are commonly targeted for phage research.

Bioremediation

The process of using microorganisms to breakdown pollutants in the environment. Gordonia terrae is attractive for bioremediation due to its ability to breakdown hydrocarbons.

Industrial biotechnology

The application of biological processes and organisms for the production of useful products in industries. Gordonia terrae is used in industrial biotechnology for its ability to breakdown phthalic acid esters and remove sulfur from fossil fuels.

Bacterial lawn

A dense layer of bacterial cells grown on an agar plate. When preparing a bacterial lawn, a large number of bacterial cells are added to promote rapid growth and colony formation.

Colony

A visible mass of bacterial cells that have grown and divided on an agar plate. It typically takes about 3 days for an individual cell to form a colony of G. terrae on an agar plate.

Cycloheximide

An antifungal compound that can be added to the growth media to prevent the growth of other microorganisms in cultures of G. terrae.

Phage isolation

The process of isolating and studying bacteriophages, viruses that infect bacteria. G. terrae is commonly used as a host for phage isolation in research.

Growth media

The nutrient-rich media on which G. terrae is grown. In this case, PYCa media is used for the growth of G. terrae.

Phage Buffer

A specific buffer solution used for the storage and handling of bacteriophages. It contains Tris, MgSO4, NaCl, and CaCl2, and can optionally include gly