Ch. 1 Microscopy

0.0(0)
Studied by 0 people
call kaiCall Kai
Locked
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
GameKnowt Play
Card Sorting

1/12

encourage image

There's no tags or description

Looks like no tags are added yet.

Last updated 2:03 AM on 9/15/26
Name
Mastery
Learn
Test
Matching
Spaced
Call with Kai
Chat

No analytics yet

Send a link to your students to track their progress

13 Terms

1
New cards

histology

study of microscopic structure and function of tissues

2
New cards

Formation of Organ Systems

Cells → Tissues → Organs → Organ Systems

3
New cards

4 types of tissues

  • epithelial

  • connective

  • muscle

  • nervous


4
New cards

Fixation

cross-linking fixatives perserve tissue before embedding

5
New cards

Common fixatives

glutaraldehyde and osmium tetroxide (EM)

paraformaldehyde (LM)

6
New cards

Prep order of a biopsy

Fixation → dehydration → cleaning → infiltration → embedding

7
New cards

sectioning tool

microtome or cryostate

8
New cards

Types of Staining

  • histological: genetic tissue characteristic

  • electron microscopy “stains”: heavy metal (osmium tetroxide embedded means electrons cannot pass)

  • immunohistochemical: specific molecule


9
New cards

Hematoxylin

basic dye, stains basophilic components blue because of GAGs and nucleic acids

10
New cards

Eosin

acid dye, stains acidophilic tissue components red/pink

  • mitochondria, secretory granules, and collagen


11
New cards

Transmission Electron Microscopy (TEM)

powerful imaging technique that shoots a beam of electrons through an ultra-thin sample to view its internal structures at the atomic level

  • lipid bilayer appears black due to osmium tetroxide (EM Stain)


12
New cards

Scanning Electron Microscope (SEM)

beam scans tissue surface 3D using electrons

  • EM stain = gold (coats specimen)


13
New cards

Sectioning Terms

  • Cross (transverse) section” 90 degree angle

  • longitudinal (horizontal) section - follows long axis

  • oblique section: angle of arm


<ul><li><p><span style="color: rgb(253, 235, 14);">Cross (transverse) section” 90 degree angle</span></p></li><li><p><span style="color: rgb(7, 247, 65);">longitudinal (horizontal) section - follows long axis</span></p></li><li><p><span style="color: rgb(255, 17, 222);">oblique section: angle of arm</span></p></li></ul><p></p>