Bacterial Transformation and Polymerase Chain Reaction

What is the clinical significance of bacterial transformation in biotechnology?

Explains antibiotic resistance, spread, enables genetic engineering for insulin production, underlies gene therapy vectors used in treating inherited diseases

When did Griffith discover transformation?

1928

How did the mouse die in station 1?

from pneumonia from the live S-strain (encapsulated) bacteria

What did the S-strain contain?

encapsulated virulent bacteria

How was the R'-strain different from the S-strain?

The R strain lacks a capsule and looks rough

What happened in station 3 of Griffith’s experiment?

the mouse survives because the heat killed bacteria were dead and couldn’t replicate

What happened in station 4?

the mouse dies from pneumonia caused by live S-strain bacteria

How did the bacteria demonstrate genetic variation?