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Hydroxyl
-OH
Polar, hydrogen bonding
Sugars, Alcohols
Carbonyl
C=O
Polar, reactive
Aldehydes, ketones
Carboxyl
-COOH
Acidic, donates H⁺
Amino acids, fatty acids
Amino
–NH₂
Basic, accepts H⁺
Amino acids
Phosphate
–PO₄²⁻
Negatively charged, energy transfer
ATP, DNA, RNA
Methyl
–CH₃
Nonpolar, hydrophobic
DNA methylation
Sulfhydryl
–SH
Forms disulfide bonds
Proteins (cysteine)