Plant prop definitions

Callus

an unorganized proliferate mass of differentiated plant cells, a wounded response.

Chemically defined medium

a nutritive solution for culturing cells in which each component is specifiable and ideally of know chemical structure

Clone

plants produced asexually from a single source plant

Clone propagation

asexual reproduction of plants that are considered to be genetically uniform and originated from a single individual or explant

Contamination

being infested with unwanted microorganisms such as bacteria or fungiC

Culture

a plant growing in vitro

Explant

tissue taken from its original site and transferred to an artificial medium for growth or maintenance

horizontal laminar flow unit

an enclosed work area that has sterile air moving across it.

In vivo

to be grown naturally

Medium

a nutritive solution, solid or liquid, for culturing cells

Micropopagation

in vitro clonal propagation of plants from shoot tips or nodal explants, usually with an accelerated proliferation of shoots, during subcultures

Passage

the transfer of transplantation of cells or tissues with or without dilution or division, form one culture vessel to another

Pathogen

a disease-causing organism

Stage 1

a step in in vitro propagation characterized by the establishment of an aseptic tissue culture plant

Stage 2

rapid numerical increase of organs of other structures

Stage 3

preparation of propagules for successful transfer to soil, a process involving rooting of shoot cuttings, hardening of plants, and initiating the change from the heterotrophic to the autotrophic state

Step 4

establishment in soil of a tissue culture derived plant, either after undergoing a stage 3 pretransplant treatment

Sterile techniques

the practice of working with cultures in an environment free from microorganisms

Tissue culture

the maintenance or growth of tissue, in vitro, in a way that may allow differentiation and preservation of their function

Totipotency

a cell characteristic in which the potential for forming all the cell types in the adult organism are retained