Studied by 0 people
Looks like no one added any tags here yet for you.
Callus
an unorganized proliferate mass of differentiated plant cells, a wounded response.
Chemically defined medium
a nutritive solution for culturing cells in which each component is specifiable and ideally of know chemical structure
Clone
plants produced asexually from a single source plant
Clone propagation
asexual reproduction of plants that are considered to be genetically uniform and originated from a single individual or explant
Contamination
being infested with unwanted microorganisms such as bacteria or fungiC
Culture
a plant growing in vitro
Explant
tissue taken from its original site and transferred to an artificial medium for growth or maintenance
horizontal laminar flow unit
an enclosed work area that has sterile air moving across it.
In vivo
to be grown naturally
Medium
a nutritive solution, solid or liquid, for culturing cells
Micropopagation
in vitro clonal propagation of plants from shoot tips or nodal explants, usually with an accelerated proliferation of shoots, during subcultures
Passage
the transfer of transplantation of cells or tissues with or without dilution or division, form one culture vessel to another
Pathogen
a disease-causing organism
Stage 1
a step in in vitro propagation characterized by the establishment of an aseptic tissue culture plant
Stage 2
rapid numerical increase of organs of other structures
Stage 3
preparation of propagules for successful transfer to soil, a process involving rooting of shoot cuttings, hardening of plants, and initiating the change from the heterotrophic to the autotrophic state
Step 4
establishment in soil of a tissue culture derived plant, either after undergoing a stage 3 pretransplant treatment
Sterile techniques
the practice of working with cultures in an environment free from microorganisms
Tissue culture
the maintenance or growth of tissue, in vitro, in a way that may allow differentiation and preservation of their function
Totipotency
a cell characteristic in which the potential for forming all the cell types in the adult organism are retained
