exam 3 competency

what is the difference b/w the procedure of a TSI vs Urease test?

TSI: stab the butt and zigzag the slant
Urease: just zigzag the slant

what is the difference b/w aerococcus and lactobacillus?

Aerococcus = GPC
Lactobacillus = GPR

TSI, Urease, and LIA reactions for salmonella

TSI: K/A, H2s+, G
LIA: K/K, H2S+ (purple slant, purple butt, blackening)
Urease: yellow (negative)

TSI, Urease, and LIA reactions for Shigella

TSI: K/A
LIA: K/A (purple slant/yellow butt)
Urease: yellow (negative)

TSI, Urease, and LIA reactions for the Proteus spp

TSI: K/A, H2s+, G
LIA: R/A (vulgaris) or R/R (mirabilis) {R=red}
Urease: pink (positive)

TSI, Urease, and LIA reactions for E. coli

TSI: A/A, G or K/A, G
LIA: K/K (purple slant/purple butt) or K/N (purple slant/neutral butt)
Urease: yellow (negative)

colony morphology of Proteus

swarming motility, smooth, pale or colorless (NLF) colonies.

colony morphology of pseudomonas

large, opaque, flat colonies with irregular margins and distinctively fruity odour colonies (grape)

what biochemical test/result identifies pseudomonas

produces non-fluorescent, blue-green, water soluble, diffusible pigment called pyocyanin

when is a urine culture acceptable?
when is it contaminated?

contaminated: >3 organisms growing and if the ratio of the organisms is not equal (one is predominant over than the other)