what is the difference b/w the procedure of a TSI vs Urease test?
TSI: stab the butt and zigzag the slant Urease: just zigzag the slant
what is the difference b/w aerococcus and lactobacillus?
Aerococcus = GPC Lactobacillus = GPR
TSI, Urease, and LIA reactions for salmonella
TSI: K/A, H2s+, G LIA: K/K, H2S+ (purple slant, purple butt, blackening) Urease: yellow (negative)
TSI, Urease, and LIA reactions for Shigella
TSI: K/A LIA: K/A (purple slant/yellow butt) Urease: yellow (negative)
TSI, Urease, and LIA reactions for the Proteus spp
TSI: K/A, H2s+, G LIA: R/A (vulgaris) or R/R (mirabilis) {R=red} Urease: pink (positive)
TSI, Urease, and LIA reactions for E. coli
TSI: A/A, G or K/A, G LIA: K/K (purple slant/purple butt) or K/N (purple slant/neutral butt) Urease: yellow (negative)
colony morphology of Proteus
swarming motility, smooth, pale or colorless (NLF) colonies.
colony morphology of pseudomonas
large, opaque, flat colonies with irregular margins and distinctively fruity odour colonies (grape)
what biochemical test/result identifies pseudomonas
produces non-fluorescent, blue-green, water soluble, diffusible pigment called pyocyanin
when is a urine culture acceptable? when is it contaminated?
contaminated: >3 organisms growing and if the ratio of the organisms is not equal (one is predominant over than the other)