Practical: Investigating Enzyme activity

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What does amylase catalyse the breakdown of?

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Amylase catalyses the breakdown of starch to maltose.

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What is the color change in iodine solution when starch is present?

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Iodine solution changes from browny-orange to blue-black when starch is present.

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10 Terms

1
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What does amylase catalyse the breakdown of?

Amylase catalyses the breakdown of starch to maltose.

2
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What is the color change in iodine solution when starch is present?

Iodine solution changes from browny-orange to blue-black when starch is present.

3
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At what temperature should the water be heated for the experiment?

The water should be heated to 35°C.

4
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What solution is used to change the pH in the experiment?

A buffer solution is used to change the pH.

5
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How long should the amylase and buffer solutions be allowed to sit before adding starch?

They should sit for 5 minutes.

6
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What is the purpose of using continuous sampling in this experiment?

To record how long it takes for the amylase to break down all of the starch.

7
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What indicates that starch is no longer present in the boiling tube?

The iodine solution remains browny-orange.

8
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What should be done when repeating the experiment with different pH values?

Control any variables, such as concentration and volume of amylase solution, for a fair test.

9
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How can the rate of reaction be calculated in this experiment?

Rate (s^-1) = 1000/time (s).

10
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What is the purpose of a pH meter in this experiment?

To accurately measure the pH of the solution.