MYCOBACTERIUM MOCK QUIZ

GRAM POSITIVE OR GRAM NEGATIVE. MYCOBACTERIA SPECIES

GRAM POSITIVE

TRUE OR FALSE. MYCOBACTERIA SPECIES ARE GRAM (+), ACID-FAST, SLENDER, THIN COCCI

FALSE

FOR PATHOGENIC MYCOBACTERIA SPECIES, THEY REQUIRE HOW MANY DAYS FOR IT TO SHOW EVIDENT GROWTH IN A CULTURE MEDIA?

MORE THAN 5 DAYS

SINCE MYCOBACTERIA SPECIES REQUIRE HIGH PROTEIN CONTENT, WHAT DO THEY NEED FOR GROWTH?

WHOLE EGG

NON PATHOGENIC MYCOBACTERIUM SPECIES ARE WHAT KIND OF GROWERS?

RAPID GROWERS

WHAT MYCOBACTERIA SPECIE IS A NON-CULTURABLE IN VITRO?

MYCOBACTERIUM LEPRAE

WHAT IS USED TO CULTURE MYCOBACTERIUM LEPRAE?

FOOTPADS OF ARMADILLO OR FOOTPADS OF MOUSE

SINCE MYCOBACTERIUM SPECIES HAVE A HIGH LIPID-CONTAINING CELL WALL, THEY ARE REGARDED AS?

ACID-FAST

TRUE OR FALSE. WHEN MYCOBACTERIUM SPECIES ARE STAINED, THEY ARE MORE DIFFICULT TO DECOLORIZE ESPECIALLY WITH THE USE OF ACID ALCOHOL

TRUE

MYCOBACTERIUM TUBERCULOSIS IS ALSO KNOWN AS?

KOCH’S BACILLUS OR TUBERCLE BACILLUS

M. TUBERCULOSIS WAS FIRST DESCRIBED BY?

ROBERT KOCH (1882)

WHAT IS THE INCLUSION BODY OF M. TUBERCULOSIS

MUCH GRANULES

COLONY APPEARANCE OF MYCOBACTERIUM TUBERCULOSIS

CAULIFLOWER LIKE

THE COLONY APPEARANCE OF M. TUBERCULOSIS IS EVIDENT WHEN INCUBATED AT WHAT TEMP?

35-37C

ASIDE FROM M. TUBERCULOSIS BEING OBLIGATE AEROBE, THEY ALSO REQUIRE ______ FOR GROWTH

5-10% CARBON DIOXIDE

THIS IS THE PRINCIPAL AGENT OF TUBERCULOSIS IN MAN

MYCOBACTERIUM TUBERCULOSIS

MODE OF TRANSMISSION OF M. TUBERCULOSIS

INHALATION OF VIABLE BACILLI

ONLY ____ OF INFECTED INDIVIDUAL DEVELOPS A DISEASE

15-20%

WHAT DISEASE REMAINS AS ONE OF THE MOST LEADING CAUSE OF MORTALITY AND MORBIDITY WORLDWIDE

TUBERCULOSIS

______ OF THE WORLD’S POPULATION IS INFECTED WITH M. TUBERCULOSIS

1/3

WHAT IS THE VIRULENCE FACTOR OF M. TUBERCULOSIS

CORD FACTOR

CORD FACTOR IS ALSO KNOWN AS?

SERPENTINE FACTOR

THE VIRULENCE FACTOR OF M. TUBERCULOSIS IS MADE UP OF?

TREHALOSE DIMYCOLATE

THIS VIRULENCE FACTOR INHIBITS LEUKOCYTE MIGRATION

CORD FACTOR

WHAT DO YOU CALL WHEN A WBC MIGRATES TO THE SITE OF INFECTION

CHEMOTAXIS

CORD FACTOR ACTIVATES ________ CREATING ________

MACROPHAGE; GRANULOMA FORMATION

WHAT DO YOU CALL THE SCAR FORMATION CAUSED BY M. TUBERCULOSIS

GHON LESIONS

CORD FACTORS INDUCES RELEASE?

TUMOR NECROSIS FACTOR ALPHA (TNF-ALPHA)

CORD FACTOR CAUSES MYCOBACTERIUM TUBERCULOSIS TO HAVE ______ UNDER THE MICROSCOPE

PARALLEL ARRANGEMENT

SULFATIDE INHIBITS WHAT?

PHAGOLYSOSOMAL FUSION

THIS IS USUALLY SEEN IN CHILDREN AND PATIENTS WHO HAS INITIAL EXPOSURE

PRIMARY TUBERCULOSIS

A PRIMARY TUBERCULOSIS THAT HAS NO APPARENT SIGNS AND SYMPTOMS, NOT INFECTIOUS

LATENT TUBERCULOSIS

A PRIMARY TUBERCULOSIS THAT OCCURS WHEN IMMUNE SYSTEM IS SUPPRESSED

REACTIVATION TUBERCULOSIS

THIS IS WHEN PATIENTS WHO ACQUIRED TUBERCULOSIS FROM ANOTHER INDIVIDUAL WITH TUBERCULOSIS

SECONDARY TUBERCULOSIS

MILITARY TUBERCULOSIS IS ALSO KNOWN AS?

MENINGEAL TB, DISSEMINATED TB

MILITARY TUBERCULOSIS IS A TYPE OF WHAT TB?

EXTRA PULMONARY TUBERCULOSIS

TUBERCULOSIS OF THE SPINE

POTT’S DISEASE

WHAT IS THE OTHER TERM FOR POTT’S DISEASE

TUBERCULAR SPONDYLITIS, SKELETAL TB

PRIMARY ANTI-TB DRUGS

RIFAMPICIN, ISONIAZID, PYRAZINAMIDE, ETHAMBUTOL (STREPTOMYCIN)

WHAT IS/ARE THE MOST COMMON DRUG REGIMEN IN PRIMARY ANTI-TB DRUGS

RIFAMPICIN AND ISONIAZID

WITH RIFAMPICIN AND ISONIAZID HOW LONG WILL THE TREATMENT FOR M. TUBERCULOSIS TAKE?

9 MONTHS

ADDITIONAL OF PYRAZINAMIDE IN RIFAMPICIN AND ISONIAZID CAN SHORTEN THE TREATMENT DOWN TO HOW MANY MONTHS?

6 MONTHS

TRUE OR FALSE. ADDING ETHAMBUTOL AND STREPTOMYCIN ALONG WITH RIFAMPICIN, ISONIAZID, PYRAZINAMIDE CAN SHORTEN THE TREATMENT TIME FOR 3 MONTHS

FALSE

WHAT ARE THE SECOND LINE ANTI-TB DRUGS

AMIKACIN, KANAMYCIN, CAPREOMYCIN

WHAT ARE THE THIRD-LINE ANTI-TB DRUGS

RIFABUTIN, MACROLIDES, LINEZOLID

MDR-TB ARE USUALLY RESISTANT TO?

RIFAMPICIN AND ISONIAZID

XDR-TB SHOWS RESISTANCE AGAINST

ISONIAZID, RIFAMPICIN, FLUOROQUINONES

WHAT KIND OF TUBERCULOSIS SHOWS RESISTANCE AGAINST ONE OF THE 3 SECONDARY ANTI-TB DRUGS

EXTENSIVELY DRUG RESISTANT MYCOBACTERIUM TUBERCULOSIS

WHAT KIND OF TUBERCULOSIS SHOWS RESISTANCE TO AT LEAST TWO PRIMARY ANTI-TB DRUG

MULTIDRUG RESISTANT MYCOBACTERIUM TUBERCULOSIS

A TEST AGAINST MYCOBACTERIUM TUBERCULOSIS

TUBERCULIN SKIN TEST

TUBERCULIN SKIN TEST IS A PROTEIN EXTRACTED AND PURIFIED FROM THE ______ OF CULTURED M. TUBERCULOSIS

CELL WALL

WHAT KIND OF TEST IS TUBERCULIN SKIN TEST?

HYPERSENSITIVITY TEST

WHAT IS THE MEANING PPD

PURIFIED PROTEIN DERIVATIVES

A POSITIVE RESULT OF TUBERCULIN SKIN TEST INDICATES WHAT?

PAST EXPOSURE TO MYCOBACTERIUM TUBERCULOSIS

THIS IS A PREFERRED SKIN TEST METHOD FOR M. TUBERCULOSIS

MANTOUX TEST

HOW MUCH OF PPD IS USED IN MANTOUX TEST?

0.1ML

IN MANTOUX TEST, PPD IS INJECTED ________ AND REACTION IS OBSERVED AFTER _______

INTRADERMALLY; 48 HOURS

POSITIVE RESULT OF MANTOUX TEST

ERYTHEMA AND SWELLING

SIZE OF SWELLING IN POSITIVE REACTION OF MANTOUX TEST

2-10MM IN DIAMETER OR MORE

A SCRATCH METHOD OF TUBERCULIN SKIN TEST

VON PIRQUET TEST

THIS METHOD OF TUBERCULIN SKIN TEST IS PERFORMED AMONG INFANTS

VOLLMER’S TUBERCULIN PATCH TEST

THIS METHOD OF TUBERCULIN SKIN TEST IS A MULTI-PUNCTURE METHOD

TUBERCULIN TINE TEST

TUBERCULIN TINE TEST USES _____ WHICH IS COVERED/COATED WITH _______

4 TINES; DRIED PPD

MYCOBACTERIUM BOVIS CAUSES TB IN?

CATTLE, DOGS, CATS, SWINE, PARROTS, AND HUMAN

MYCOBACTERIUM BOVIS IS ASSOCIATED WITH?

INGESTION OF UNPASTEURIZED COW’S MILK

WHICH IS A SOURCE OF TB VACCINE FOR HUMAN

MYCOBACTERIUM BOVIS

WHAT DOES BCG VACCINE MEAN?

BACILLUS OF CALMETTE AND GUERIN VACCINE

M. BOVIS IS A SOURCE OF TB VACCINE BECAUSE M. BOVIS AND M. TUBERCULOSIS SHOWS SIMILAR?

ANITGENIC DETERMINANTS

M. BOVIS GROWS AT WHAT TEMP?

35-37C

MEDIA FOR M. BOVIS

MIDDLEBROOK MEDIA AND LOWENSTEIN JENSEN MEDIA

IN MIDDLEBROOK MEDIA, M. BOVIS IS EVIDENT FOR HOW LONG?

3 WEEKS

IN LJ MEDIA, M. BOVIS IS EVIDENT FOR HOW LONG?

6-8 WEEKS

IN LJ MEDIA, WHAT IS THE APPEARANCE OF M. BOVIS?

WATER DROPLET APPEARANCE

IN WHAT TEMP DOES M. BOVIS SHOWS NO GROWTH?

24-42C

WHAT TEST CAN HELP DIFFERENTIATE M. BOVIS FROM M. TUBERCULOSIS?

NIACIN TEST, NITRATE REDUCTION TEST, PYRAZINAMIDASE TEST

IN NIACIN TEST, NITRATE REDUCTION TEST, PYRAZINAMIDASE TEST WHICH BACTERIA SHOWS NEGATIVE REACTION IN ALL THESE TEST?

MYCOBACTERIUM BOVIS

A BACTERIA ASSOCIATED WITH TB IN TROPICAL AFRICA

MYCOBACTERIUM AFRICANUM

TUBERCULOSIS THAT IS SEEN IN CHILDREN WITH AIDS

MYCOBACTERIUM CANETTII

TUBERCULOSIS IN BOTH IMMUNOCOMPETENT AND IMMUNOCOMPROMISED INDIVIDUAL

MYCOBACTERIUM MICROTI

WHAT IS THE AGENT OF TUBERCULOSIS IN ANIMALS

MYCOBACTERIUM MICROTI

IT IS/ARE THE FIRST ISOLATED FROM ANIMALS BUT MAY ALSO BE TRANSMITTED TO HUMAN

MYCOBACTERIUM PINNIPEDII AND MYCOBACTERIUM CAPRAE

WHERE CAN MOTT BE FOUND?

ENVIRONMENT

MOTT ARE GROUPED BASED ON?

RUNYOUN’S CLASSIFICATION

THIS SHOWS YELLOW PIGMENT WHEN EXPOSED TO CONSTANT LIGHT

GROUP 1 - PHOTOCHROMOGEN

THIS SHOWS YELLOW-TO-ORANGE PIGMENT IN DARK AND UNDER LIGHT

GROUP 2 - SCOTOCHROMOGEN

THEY ARE NON PIGMENTED BOTH IN DARK AND LIGHT

GROUP 3 - NON-PHOTOCHROMOGEN

SINCE GROUP 4 RAPID GROWERS HAVE VARYING PIGMENTATION THEIR KEY CHARACTERISTICS IS THAT THEIR GROWTH RATE IS AT?

3-5 DAYS

WHICH OF THE MOTT HAVE A GROWTH RATE OF 10-21 DAYS

PHOTOCHROMOGEN, SCOTOCHROMOGEN, NON-PHOTOCHROMOGEN

WHAT BACTERIA IS ALSO KNOWN AS THE YELLOW BACILLUS

MYCOBACTERIUM KANSASII

M. KANSASII PRODUCES YELLOW PIGMENT WHEN EXPOSED TO LIGHT DUE TO THE PRESENCE OF?

BETA-CAROTENE

A DERIVATIVE OF VITAMIN A THAT CAN BE FOUND IN YELLOW BACILLUS

BETA-CAROTENE

YELLOW PIGMENT OF MYCOBACTERIUM KANSASII IS ONLY EVIDENT ON?

YOUNG CULTURE

IF PROLONGED INCUBATION ON EXPOSED LIGHT OF M. KANSASII, THE PIGMENT MAY CHANGE TO WHAT COLOR?

RED

WHAT IS THE SECOND COMMON CAUSE OF NONTUBERCULOUS MYCOBACTERIUM LUNG DISEASE AMONG AIDS PATIENT

MYCOBACTERIUM KANSASII

APPEARANCE OF YELLOW BACILLUS WHEN SEEN IN MICROSCOPE

SHEPHERD’S HOOK OR CANDY CANE APPEARANCE

M. KANSASII GROWS WELL AT WHAT TEMP?

37C

GROWTH OF YELLOW BACILLUS IS EVIDENT AFTER HOW MANY DAYS?

14-28 DAYS

SLOW GROWTH OF YELLOW BACILLUS IS SEEN IN WHAT TEMP?

24C

M. KANSASII IS RAPID TWEEN 80 HYDROLYSIS POSITIVE BECAUSE THEY CAN HYDROLYZE TWEEN 80 WITHIN?

3 DAYS

MOST BACTERIA CAN HYDROLYZE TWEEN 80 WITHIN?

10 DAYS