Lecture 35 Nucleotide Metabolism and the Pentose Phosphate Pathway

The difference between a nucleotide and a nucleoside is a ________ _____

phosphate group

_____ is produced during the pentose phosphate pathway and is required for lipid metabolism and nucleotide synthesis.

NADPH

______ is produced via the pentose phosphate pathway and is important in the synthesis of nucleotide.

Ribose

__ ____ pathways synthesize nucleotides from scratch, and do not use free bases.

De novo

___ is the most abundant nucleotide in the cell.

ATP

The committed step of de novo synthesis (purines and pyrimidines) is the combination of _________ with PRPP.

glutamine

_____ ____ is important in de novo purine synthesis, as well as _____________.

Folic acid; methotrexate

The precursor for purines (AMP and GMP) is ___ which contains ____________

IMP (inosine monophosphate); hypoxanthine

Nucleosides are chain terminating because they lack a __ ________ _____

3' hydroxyl group

Enzymes to form ____________ are base specific.

diphosphates

Enzymes to form _____________ are non-specific.

triphosphates

Nucleotides need to be ____________ to be used by DNA polymerase.

triphosphates

___-_____ have their own kinase which allows them to become a triphosphate and be incorporated into DNA, and terminate the chain.

Pro-drugs

____ regulates nucleotide synthesis.

PRPP

______ synthesis is regulated by the final product.

Purine