2.1.1 Use of apparatus and techniques

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8 Terms

1
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When would you use colorimetry?
Method to measure substance concentration in a solution by analyzing light absorbance/transmission of a specific wavelength. Used in chemistry and biochemistry to analyze sample substance concentration.
2
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When would you use a potometer?
Device used to measure the rate of transpiration in plants. It is used to investigate the effect of environmental factors on the rate of water uptake by a plant.
3
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When would you carry out serial dilution?
A method to create lower concentrations of a concentrated solution, often used in microbiology to measure bacteria concentration.
4
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When would you use electrophoresis?
A laboratory technique that separates DNA, RNA or proteins based on their size and charge. It is used for DNA fingerprinting, disease diagnosis, and drug development.
5
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When would you use thin layer/paper chromatography?
Separation technique used to identify and quantify small organic molecules in a mixture.
6
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How do you ensure the safe use of instruments for the dissection of animals or an organ?
* Wear personal protective equipment (PPE)


* Keep sharp instruments pointed away from yourself and others
* Dispose of used instruments in a sharps container
* Clean instruments with appropriate disinfectant after use.
7
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When do you use which sampling techniques in field work?
Systematic sampling is used when a pattern is present in the population. Cluster sampling is used when the population is geographically dispersed. Stratified sampling is used when the population can be divided into subgroups based on a characteristic of interest. Random sampling is used when the population is homogenous and there is no pattern present.
8
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How do you ensure the safe and ethical use of organisms in school practicals?
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