Phlebotomy study set

CLSI order of draw chart

Blood culture, discard tube, light blue top, rep top, gold/tiger/marble, light green top, dark green top, pink top, lavender top, gray top, dark blue

discard tube color:

clear or red

blood culture bottles additive

Nutrient broth

blood culture testing purpose:

for systemic infection or fever of unknown orgin (FUO). testing is done in microbiology

blood culture collection method:

aerobic (w/ oxygen) and anaerobic (w/o oxygen)

clear tube top purpose:

to discard. to avoid thrombin and other cells from polluting the samples drawn into different tubes.

light blue top additive

sodium citrate (anticoagulant)

light blue top testing testing purpose:

Prothrombin time (pt), partial thrombin time (ppt), activated thrombinplastin time (appt)

platelet rich plasma therapy (PRP)

blood is drawn in two light blue tubes

light blue tubes are inverted ___

3-4 times

light blues top tube ratio

9 parts blood and 1 part additive

prothrombin time test purpose

evaluates the extrinsic system of the coagulation cascade. therapeutic drug monitoring requires prothrombin time to be drawn when a patient is on a coumadin regimen .

partial thromboplastin time

evaluates the intrinsic system of the coagulation cascade. therapeutic drug monitoring requires partial thromboplastin time to be drawn when a patient is on a heparin regimen.

activated partial thromboplastin time

evaluates how long the patient’s take to form a clot.

red top tube glass additive

none

red top tube plastic additive

clot activator

red top tube is inverted ____

0

red top tube testing purpose:

can be used for a serum pregnancy test

tiger, marble, red-gray spotted, gold top tube additive

silica, (clot activator) and thixotropic gel (serum separator)

silica purpose

promotes rapid clot formation of the blood (30 minutes)