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The Scientific Method was created by…
Sir Francis Bacon (1561-1926)
The Scientific Method
A process or steps taken to produce correct and trustworthy results to answer a specific question.
Steps of the Scientific Method
Observation
Research
Hypothesis
Experiment
Analyze Results
Share
Repeat
Observation
All that can be observed with the 5 senses
Research
Using reliable sources to learn background information
Reliable Sources
Scientific journals, online sources that are vetted and trusted
Unreliable Sources
Blogs, out-of-date textbooks
Hypothesis
What you think will occur, “If then BECAUSE…”
Experiment
Qualitative and Quantitative Data
Independent and Dependent Variables
Experimental Group and Control Group
Qualitative Data
Data that relies on descriptions
Quantitative Data
Data that relies on numbers
Independent Variable
The thing you change
Dependent Variable
The thing you measure
Experimental Group
Has one independent variable altered
Control Group
All other variables are compared to this (normal)
Atom
The basic unit of matter
Covalent Bond
One or more electrons are SHARED between atoms
Ionic Bond
One or more electrons are TRANSFERRED from 1 atom to another
Cation
Positive ion, an electron is given away by an element
Anion
Negative ion, an electron is taken in by an elemt
Vanderwaals Forces
A temporary electrostatic attraction can develop between 2 polarized molecules
Hydrogen Bonding
Because of their partial positive and negative charges, hydrogen can form WEAK attractions with other elements. However this is NOT AN ACTUAL BOND.
Fluorine, Oxygen, and Nitrogen
Elements that can interact with Hydrogen
Water Polarity
A water molecule is polar due to the uneven pull of electrons between oxygen and hydrogen
Cohesion
Same substances binding with each other, water is extremely cohesive.
Adhesion
Molecules of different substances attract to each other
Capillary Action
Water defying gravity by binding with other substances (type of adhesion)
Surface Tension
When the hydrogen bond between water molecules is strong enough to form a thin film and have a specific shape
Peptide Bonds
Chain of Amino Acids
Carbohydrates
Elements: CHO, 1:2:1 ratio
Function: Main source of energy and structural support (cellulose & chitin)
Monomer & General Shape: Monosaccharides are it’s monomer, it’s general structure is a ring/rings
Examples: Sugars, starches, and glucose
Lipid
Elements: CHO
Function: Long term storage of energy, important component of membranes, hormones (testosterone, estrogen, and cortisol)
Monomer & General Shape: It’s monomers include fatty Acids (tails/chains), glycerol (head), tryglyceride and it’s general structure are chains
Examples: Butter, saturated and unsaturated fatty acids
Protein
Elements: CHON
Function: Produce enzymes to control reactions, forms muscles and bones, transports substances in and out of cells, and fights diseases
Monomer & General Shape: It’s monomers are amino acids and it’s general structure consists of the Amine Group, Carboxyl Group, and R-Group (Spare Group)
Examples: Enzymes, antibodies, cell membrane proteins, structural - hair/nails, muscle etc.
Nucleic Acids
Elements: CHOPN
Function: Stores and transmits genetic information
Monomer & General Shape: It’s monomers are nucleotides and it’s gneeral structure consists of 5-Carbon Sugar (Pentose), the Phosphate Group, and a nitrogenous base
Examples: DNA and RNA
What makes substances organic?
Carbon
Saturated Fats
Single bonds to allow them to stack which is “bad”
From animal sources
Solid at room temperature
Unsaturated Fats
Double bonds which bends
Can not stack which is “good”
Mostly derived from plant sources
Liquid at room temperature
Dehydration Synthesis
Definition: Monomers are joined together to form polymers
Can be identified in equations if a “+ H20” is put after the polymer
Dehydration - Remove water
Synthesis - Put together
Hydrolysis
Definition: Water acts like a catalyst and breaks the bonds between 2 macromolecules
Hydro - Water
Lysis - Breaks apart
1-6 pH
Acidic, taste sour
7 pH
Neutral, pure water
8-14 pH
Basic, taste bitter
Acids have a larger concentration of…
Hydrogen ions (H+)
Bases have a larger concentration of…
Hydroxide ions (OH-)
The eyepiece lens is located in…
the top portion of the microscope
What magnification does the eyepiece lens usually have?
10x
Longer lenses mean…
higher power
Carry the microscope by placing one hand beneath the ____ and grasping the ___ of the microscope with the other hand
base, arm
Gently place the microscope on a lab table with the arm facing _______ you
towards
The base of the microscope should be resting ______ on the lab table, approximately __ cm away from it’s edge.
evenly, 10
Raise the ____ ____ by turning the ______ adjustment knob first until the ________ lens is _ cm away from the ____
body tube, coarse, scanning, 2, stage
Rotate the _________ so the scanning objective lens is directly in line with the stage opening.
nosepiece
Look through the eyepiece and switch on the ____ so a circle of light can be seen.
lamp
Field of View
The circle of light that can be seen when the lamp is switched on
Move the _________ to let more or less light come through the stage opening.
diaphragm
Place a prepared slide on the stage so the specimen is over the center of the opening. Use _____ _____ to hold it in place.
stage clips
Look at the microscope from the ____.
side
Do not lower the body tube with the coarse adjustment knob while…
looking through the eyepiece!
Use the ____ adjustment knob after focusing the image the best you can with coarse objective.
fine
Why should you keep both eyes open when viewing the specimen?
To prevent eyestrain
To change _____________, rotate the nospiece until the desired objective lens clicks into place.
magnification
After every use…
remove the slide, put the lens back to scanning objective and clean the stage of the microscope and lenses with lense paper.
Why should you not use paper other than lense paper to clean the microscope lense?
Because they may scratch the lenses?
How to create a wet mount
Use a clean microscope slide and coverslip
Place the specimen in the middle of the microscope slide
Use a dropper pipette (eye dropper) to place 1-3 drops. of ater on the specimen depending on it’s size
Slowly lower one edge of the coverslip so that it touches a side of the drop of water
Remove any excess water with a paper towel
What angle should the coverslip make with the drop of water when creating a wet mount?
45 degree angle
How should you lower the coverslip in a wet mount?
slowly, with a dissecting needle or probe
What should you do if air bubbles are present in a wet mount?
Gently tap the surface of the coverslip over the air bubbles with a pencil eraser
What should you do if the specimen begins to dry out in a wet mount?
Add a drop of water to the edge of the coverslip
Why are wet mound slides necessary?
They make it easier to get a clearer image of our specimen by increasing their translucency, they also make it easier to stain a specimen.
In a wet mound slide, the specimen must be ____ enough for light to pass through it
thin
Parts of the traditional cell theory and the scientists who are credited with them
All living things are made of cells, Theodore Schwann and Matthias Schleiden
Cells are the basic unit of structure and function in living things, Theodore Schwann and Matthias Schleiden
New cells are produced from existing cells, Rudoph Virchow (Robert Remak)
Modern additions to the cell theory
Energy flow occurs within cells
Genetic info (DNA) is passed from one cell to another during cell division
All cells have the same basic chemical composition in organisms of similar species
Nucleus
Control center of cell, largest organelle in the cell and contains most of the DNA of the cell, stores DNA
Nuclear DNA
Contain the genetic info for cells to grow, live, perform their functions and reproduce
Nucleolus
Responsible for making ribosomes
Nuclear envelope
Allows substances made in the nucleus to pass into cytoplasm
Centrioles
Help make cytoskeleton
Golgi bodies (complex)
Sort and ship the proteins produced in the ER. Proteins are processed and packaged into new vesicles here.
Rough Endoplasmic Reticulum
Ribosomes are attached to it. A network of membranes found in the cytoplasm where most protein synthesis occurs. A portion of internal membranes.
Smooth Endoplasmic Reticulum
Makes lipids in the cell, helps to process or detoxify harmful substances found in the cell. Lacks ribosomes, portion of internal membranes
Mitochondrion
Package the energy of food into ATP molecules, provides energy for cells.
Vesicles
“Small vessel,” helps store and transport products produced by the cell
Peroxisome
Collect and safely break down chemicals that are toxic to the cell
Lysosomes
Help break down large molecules into smaller pieces that the cell can use, eliminate waste from cells
Cytoskeleton
Helps shape and support the cell, also helps move things around the cell
Cell membrane
Seperates the inside of the cell from the rest of its environment, made of 2 layers of lipids. AKA the plasma membrane
(Protein) Channel
Made up of certain proteins that help control the movements of food and water into the cell
Cytosol
The water fluid that surrounds all organelles
Ribosomes
Use a form of DNA instructions to make proteins that the cell needs. Also make proteins that may be exported from the cell for work in other parts of the organims.
Chloroplast
Where photosynthesis takes place. Light energy from the sun is converted into chemical energy.
Chloroplast is mostly found in…
plant cells
Plant cells do not have…
centrioles and lysosomes
Cell wall
Provides protection and support for plants. Unlike cell membranes, materials cannot get through this.
Vacuole
Contains large amounts of water and stores other important materials like sugars, ions, and pigments
Plasmodesmata
Openings used to communicate and transport materials between plant cells because the cell membranes are able to touch and so they can exchange needed materials.
Flagella (Bacterium)
A tail attached to the main body of the cell that can roatet to move the cell forward
Pili (Bacterium)
Long, threadlike strings coming off of the cells surface. Bacteria can use these to bind to other bacteria cells to exchange genetic material.
Capsule (Bacterium)
A thick outer layer that protects the cell
What two protective layers does Bacterium share with plant and animal cells?
Cell Wall - Bacteria cells and plant cells
Cell membrane - Bacteria cells and plant & animal cells
Plasmid (Bacterium)
Little loops of DNA that holds extra genes. Bacteria can exchange these with others to get new genes.
Cell membranes and cell walls are…
cell boundaries