Advanced Bio: Key Terms on Restriction Enzymes

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9 Terms

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How

Recognize specific DNA base sequence -> cut DNA at recognition sequence in consisten way (restriction sites)

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Discovery

in bacteria, which used them to destroy foreign DNA

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Cut DNA pieces are called

restriction fragments

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How many?

More than 4000

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Staggered cuts create

sticky ends

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Straight cuts create

blunt ends

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Benefits of sticky ends

Can bond with complementary sticky ends on other DNA cut with same enzyme

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How can pieces be joined?

DNA ligase-> recombinant DNA

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Advantages using restriction enzymes

-made it possible to work with small and defined pieces of DNA
-study small, specific pieces of chromosomes
-recombinant DNA
-clone segments of DNA into plasmids and viruses
-manipulate specific pieces of DNA