Biochem Lab 2 Quiz

What is the assay based on?

Lowry procedure

What type of assay is being used?

Colorimetric Assay

What are the advantages of this assay?

Widely cited, modified cupric sulfate-tartrate reagent is stable at room temperature

what does the assay involve?

a reaction of protein with cupric sulfate in alkaline solution resulting in the formation of tetradentate cooper protein complexes

What do the cooper protein complexes measure at?

650 nm and normalized at 405nm.

Range of the Modified Lowry grapb

linear range from 200 to 4000 ug/mL

what is the ratio of the reagent for the sample

5:1

what type of curve is required every time an assay is run

standard curve, set a new one each time

what do you need to make sure to do with the diluent for the blanks, standards, and unknown samples

same pH and ionic strength

what is a standard

solution of known concentration that is used to calibrate an instrument

**known concentration

what fluid is used for the standard?

NanoDrop CF-1 Calibration Check Fluid

what is a control

a solution that produces an expected result within a specific range

***specific range

what is the control

2 ug/ml Bovine Serum Alubmin solution

What do negative values of absorbance mean?

pedestals are dirty when a blank was made or a sample was used to make a blank

how to fix a negative value of absorbance

clean pedestal

What does a jagged absorption mean

a broken column

what does a concentration mean if it NaN or Out of Range?

sample concentration is outside the range of the standard curve

How to fix an Out of Range sample?

make sure the standard will cover the expected range