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Name five parts of an animal cell?
Nucleus, cytoplasm, cell membrane, ribosomes, mitochondria
Name three structures found in plant cells but not in animal cells?
Chloroplast, Cell wall, Vacuole
What type of cell(s) contain plasmids?
Bacteria
Name the part of the cell that controls the activities of the cell?
Nucleus
What important substances are made by ribosomes?
Proteins
What controls the entry and exit of materials in a cell?
Cell membrane
What is the function of the cytoplasm?
Site of chemical reactions
What is the site of aerobic respiration (a chemical reaction which releases energy from food) in a cell?
Mitochondria
Which types of cell(s) have a cell wall?
Plants, Fungal and Bacterial
Where do you call the fluid-filled sac containing water, sugars and salts which is found in a plant or fungal cell?
Vacuole
What chemical reaction takes place inside a chloroplast uisng energy from the sun?
Photosynthesis
Name the pigment found in chloroplasts which trap light energy from the sun?
Chlorophyll
What is a plant cell wall composed of?
Cellulose
Are plant and bacterial cell walls made of cellulose?
No - only plant
Name two type of cells other than animal and plant.
Fungal and bacterial cells
Name two ways in which bacterial cells are different to the other three types.
Bacterial cells have no nucleus and no organelles except ribosomes.
What do bacteria have instead of a nucleus?
A loop of DNA and circular plasmids.
Which two substances make up the cell membrane?
Phospholipids and proteins
What do you call the movement of molecules from a region of higher concentration to lower concentration (down the concentration gradient).
Diffusion
State two useful substances that cells gain by diffusion
Oxygen, glucose, amino acids
State two waste substances that cells lose by diffusion
Carbon dioxide, urea
What is meant by passive transport?
A type of transport that occurs down the concentration gradient and does not require energy.
What words describe the property of cell membranes whereby they allow only some substances to cross.
semi-permeable / selectively permeable
Why can starch not pass through the cell membrane by diffusion?
It is too large to fit through the cell membrane.
Define osmosis
Osmosis is the movement of water molecules from a region of higher water concentration to a region of lower water concentration through a selectively permeable membrane.
What word is used to describe a plant cell when it is placed in a solution of lower water concentration.
Plasmolysed
What do you call a plant cell that has been placed in a solution of higher water concentration?
Turgid
What happens to an animal cell when it is placed in a solution of higher water concentration?
Burst - because they have no cell wall to maintain the structure.
What kind of solution makes an animal cell shrink?
A lower water concentration.
What kinds of transport moves substances against their concentration gradient and requires energy?
Active transport.
As well as energy, what else does active transport require?
Membrane proteins
Visking tubing is often described as a model cell. Why?
It has a semi-permeable membrane
When investigating osmosis in potato cylinders, what must you do to them before you re-weigh them?
blot them dry to remove excess water
Describe the structure of DNA
Double-stranded helix held by complementary base pairs.
What is DNA made from?
A sequence of bases - A, T, C, G
What are the base pairing rules in DNA?
Adenine-Thymine, Guanine - Cytosine
Three bases together code for what chemicals that make proteins?
Amino acids
Name the molecule which carries a complementary copy of DNA to the ribosomes?
mRNA
What is a gene?
A section of DNA / sequence of bases that codes for a protein
How does DNA determine the structure of a protein?
The base sequence determines amino acid sequence; which determines the shape of the protein.
Name three functions of proteins.
Structural, enzymes, hormones, antibodies, haemoglobin
How do the variety of protein shapes arise?
The different sequences of amino acids.
What are biological catalysts called?
Enzymes
Where can you find enzymes?
In all living cells
What is the function of enzymes?
Speed up chemical reactions
What do we call the part of an enzyme that is complementary to its specific substrate(s)?
Active site
What is the word that describes the fact that each enzyme only acts on one substrate?
Specific
What is formed at the end of an enzyme controlled reaction?
Products (and unchanged enzyme)
What type of reaction is it when an enzyme builds up large molecules from more smaller molecules?
Synthesis
What type of reaction is it when an enzyme breaks down large molecules into many smaller molecules?
Degradation
What enzyme breaks down hydrogen peroxide into water and oxygen?
Catalase
Name the enzyme that breaks down starch to maltose?
Amylase
Draw a graph showing activity of an enzyme against pH, and activity of an enzyme against temperature.
What happens to enzymes at high temperatures and extremes of pH?
They are denatured (shape of the active site is permanently changed) and the rate of reaction decreases.
What word describes the conditions at which an enzyme works best?
Optimum
Name the process where genetic information (DNA) can be transferred from one cell to another
Genetic engineering
In genetic engineering what is identified at and then extracted from the source chromosome?
A gene
Name the small circular pieces of DNA that are extracted from bacterial cells in genetic engineering?
Plasmid
Name two products name using genetic engineering
Insulin, growth hormone, new antibiotics.
What types of proteins cut the gene from the DNA and then seals it into the plasmid?
Enzymes
What do you do with the modified plasmid in genetic engineering?
Put it into a host bacterial cell to produce a GM organism.
Name the process where chemical energy stored in glucose is released by a series of enzyme-controlled reactions.
Respiration
Name the high energy molecule which is regenerated in aerobic respiration.
ATP
ATP is regenerated from which two molecules
ADP and Pi
State three uses for the energy released from food during respiration
Cell division, protein synthesis, muscle contraction, generation of nerve impulses, photosynthesis
What type of respiration generates the most ATP from the full breakdown of a glucose molecule?
aerobic respiration
Write the word equation for aerobic respiration.
glucose + oxygen --> carbon dioxide + water + a large amount of ATP
What is the first stage of respiration called? Describe what happens in this stage.
Glycolysis, glucose is split into two molecules of pyruvate.
Two molecules of ATP are regenerated from ADP + Pi
Where does the first part of aerobic respiration / fermentation take place?
Cytoplasm
Where does the second stage of aerobic respiration take place?
Mitochondria
In the absence of oxygen, what respiratory pathway takes place?
Fermentation
Write the word equation for the fermentation pathway in animals and bacteria
glucose --> lactate + 2 ATP
Write the word equation for the fermentation pathway in plants or fungi
glucose --> carbon dioxide + ethanol + 2 ATP
What is a respirometer?
Apparatus that is used to measure the rate of respiration of an organism by measuring the oxygen uptake
What would be a suitable control for a respirometer?
Set up the respirometer exactly as the original experiment but use a dead version of the organism / glass beads that are the same mass as the organism.