Key Molecular Biology Techniques and Methods

DNA Amplification Techniques

PCR, qPCR (Real-Time PCR), RT-qPCR

Gene Editing & Mutagenesis Techniques

Site-Directed Mutagenesis, Random DNA Mutagenesis (Error-Prone PCR), RNA Interference (RNAi), CRISPR-Cas9 Genome Editing, Homologous Recombination (Cre-Lox)

Gene Delivery Methods

Transformation, Transduction

Protein Tagging & Fusion Techniques

Fusion Proteins with Epitope Tags, Fusion Proteins with Fluorescent Tags

Blotting & Hybridization Techniques

Southern Blot, Northern Blot, Western Blot

Microscopy & Imaging Techniques

Fluorescence Microscopy, FISH (Fluorescence In Situ Hybridization), CLIP / CLIP-Seq (Crosslinking & Immunoprecipitation), RIP (RNA Immunoprecipitation)

DNA-Protein Interaction Assays

EMSA (Electrophoretic Mobility Shift Assay), DNase I Footprinting, Modification Interference Assay, Potassium Permanganate Footprinting

Chromatin Conformation & 3D Genome Techniques

3C (Chromatin Conformation Capture) and derivatives (4C, 5C, Hi-C)

Protein Sequencing & Identification Techniques

Mass Spectrometry (Proteomics), Edman Degradation

Structural Biology Techniques

X-Ray Crystallography, NMR Spectroscopy, Cryo-EM