Pre and Post lab quiz 1

What are unwanted organisms on media or tools called? 

contaminants

Aseptic technique refers to a procedure performed under ____________ conditions

sterile

After placing all your items in a cubby, you should _______________________. This is the first aseptic technique performed in this lab. 

wash your hands

Aseptic technique when culturing requires _______________ sterilization

dry heat

How would you properly sterilize your inoculating loop/needle?

by flaming the inoculating tool

How should you check the inoculating tool is cool enough to collect a sample?

by lightly touching it to an uninoculated area on the agar plate or wait for 8 seconds

Where should you label your agar plate?

agar side, around the edge

How should you incubate your agar plate? Why?

agar side up, because of condensation

What type of microscope will we be using in lab?

brightfield

How should you properly handle and carry a microscope? (One answer is better than the others!)

One hand on the base, the other grasping the arm, and close to the body

What type of paper should you use to clean the lens of the microscope?

lens paper

When do you need to use the immersion oil?

100x objective

When considering total magnification, you must consider which lenses?

objective and ocular lens

How many streaks/quadrants should you have when completing a Streak for Isolation?

4

How many categories are there in colony morphology?

5

What must you do for your Simple stain, but not for your negative Stain?

heat fix

A simple stain is a(n) ____________ stain.

basic

• cationic, bacteria bind to the dye, and appear colored while background is colorless

A negative stain is a(n) _____________ stain.

acidic

• anionic, stain background and leave microbe transparent

How do you prepare a smear?

Draw a circle on slide, Add bacterial sample using inoculating tool or pipette inside circle, wait for sample to dry

You collect a sample and want to cultivate a variety of microorganisms; which type of media would be best to use?

complex

You have collected a sample and want to cultivate a specific bacteria that you know requires specific nutrients and environment; which media should you use?

defined

You walk into lab and no media is labeled. You see three different types of media. 

A: solid media in an upright test tube

B: solid media in a petri dish

C: solid media in an angled test tube

D: liquid media in a test tube

Which media would you label as a slant?

C

You walk into lab and no media is labeled. You see three different types of media. 

A: solid media in an upright test tube

B: solid media in a petri dish

C: solid media in an angled test tube

D: liquid media in a test tube

Which media would you label as a broth?

D

Where should the test tube cap be while you sterilize the neck of the test tube?

in your hand

How do you check for the coolness of your inoculating loop?

by touching an uninoculated edge of the media

You are working with a lab partner and notice these characteristics and techniques:

A: fully button lab coat, crocs, and long pants

B: Washed their hands and sprayed lab benchtop

C: Completely removed lid from agar plate to inoculate

D: Labeled plate on agar side and incubated agar side up

Which characteristic(s)/technique(s) were incorrect?

A and C

Why is it important to incubate your agar plate, agar side up?

avoid condensation

Which pipette should you use if you need to collect more than 1000uL of sample?

serological pipette

The work of which scientist led to the germ theory of disease?

Robert Koch

Microbes that are permanent residents on your body are _________________?

normal microbiota

What information must you include on your agar plates?

• Procedure

• initials

• section #

• BIOL 2111

• all of the above

all of the above

What is a bacterial colony?

millions of cells together

Which of the following is NOT a colony morphology characteristic?

• Arrangement

• Size

• Color

• Margin

arrangement

Soaps have anionic which contains a ________________ end and a ___________________ end.

hydrophilic; hydrophobic

Bar soaps should be the most efficient because of the ______________ and ______________ actions.

chemical and mechanical

An isolated colony of a single microorganism species is an example of a _______________ colony.

pure

The Streak for Isolations requires a total of ______ streaks to ensure isolated colonies.

4

If you measured a colony in cm and it was 0.7cm; how much does it measure in mm?

7mm

Which streak should be the most dense streak on a Streak for Isolation plate?

1st streak

The ability of lenses to show two adjacent objects as discrete entities is referred to as:

resolution

You are looking at a slide using the 4x objective lens. What is the total magnification?

40x

You are using the 100x objective lense. To get a better focus on the slide, what must you use?

immersion oil

Acidic stains are also known as _______________. 

indirect stains

Basic stains are also known as ______________. 

direct stains