Genetic Engineering and Functional Genomics

This set of flashcards covers key concepts from the lecture notes on genetic engineering and functional genomics.

Transformation was first demonstrated in __ by Frederick Griffith.

1928

Avery's Transformation Experiment was conducted in __.

1944

Conjugation is regarded as the bacterial equivalent of __.

sexual reproduction

In DNA replication in prokaryotes, speed is higher at __ bp/s.

1000

The __ box is a cis-acting element involved in transcription regulation in eukaryotes.

TATA

In gene regulation, when lactose is not abundant, the Lac genes do not need to be __.

ON

The __ cycle leads to the destruction of the infected cell and its membrane.

lytic

__ are viruses that have RNA instead of DNA as their genetic material.

Retroviruses

A __ library is a collection of the total genomic DNA from a single organism.

genomic

The __ system is a device used to deliver exogenous DNA to cells in genetic engineering.

gene gun or biolistic particle delivery system

Site-directed mutagenesis is a __ genetic approach.

reverse

RNA interference is a reverse genetic approach aiming to __ specific mRNA.

degrade

The central dogma of biology describes the flow of genetic information from __ to phenotype.

gene

E. coli is commonly used as a source of __ in gene cloning.

plasmid

Successful insertion of T-DNA results in the plant genome being __.

randomly modified

Gene A is first transcribed to __ and then translated to protein.

mRNA

The enzyme used in reverse transcription is __.

reverse transcriptase

In __ sequencing, one of the dNTPs is radio-labeled for detection.

Sanger

Quantitative real-time PCR compares the relative transcript abundance of a gene between and samples.

water-stressed; well-watered