SS- Part 1 (Lect)

Alkaline Fast Green

Basic proteins : Protamines & Histones

Peracetic Acid-Alcian Blue

Cystine and Cysteine

Sakaguchi’s Test

Arginine

Gomori – Calcium Method

Alkaline Phosphatase Activity

Gomori – Lead Method

Acid Phosphatase Activity

Wachstein & Meisel

Lead Method for 5-nucleotidase

Alpha-naphthyl Acetate Method

Nonspecific Esterase- 1

Indoxyl Acetate Method

Nonspecific Esterase- 2

Tetrazolium Method

Monoamine Oxidase

Gomori–Calcium

(+) brownish- black

Gomori-Lead

(+) black precipitate

Wachstein & Meisel

(+) blackish-brown deposit. For unfixed free floating cryostat sections. Prefixed sections are not preferred due to loss of enzyme activity

Alpha-naphthyl acetate

(+) reddish brown color due to alpha naphthol with diazonium salt which produces insoluble dye at the site of enzyme.

Holt & Withers

Indoxyl acetate other nme

Indoxyl acetate

This uses bromo-indoxyl acetate producing bromoindoxyl and oxidized to an insoluble azo dye giving the enzyme activity blue.

Tetrazolium

uses unfixed cryostat sections. The enzyme activity is demonstrated by oxidized tetra nitro-blue tetrazolium giving the activity (+) bluish black color.

Rapid Toluidine-Eosin Stain

Glycol Methacrylate Section

Wright’s-Giemsa-Jenner stain

Improves Staining Of Cytoplasmic Granules

Peroxidase Reaction

Myeloid Cells Except Basophils

Wright’s-Giemsa-Jenner

combination of Romanowsky stain w/ other stain

Peroxidase Reaction

(+) green to dark blue granules (-) Basophil, Lymphocytes, Erythroblasts

Feulgen's Technique

Nuclear DNA

Feulgen's Technique

most reliable and specific histochemical staining technique

Acridine Orange Fluorescent Staining

DNA and RNA- 2

Acridine Orange Fluorescent

most commonly used fluorochrome

technique for DNA (orange) and RNA (red) demonstration

Methyl Green-Pyronin Method

DNA and RNA- 1

Modified Gomori’s Trichrome

Muscle Fiber and Collagen

Mallory’s Phosphotungstic Acid (PTAH)

Muscle, Neuroglia, Myelin and Collagen

Heidenhain’s Iron Hematoxylin

Muscle striations, Mitochondria, Myelin & Chromatin

Lissamine Fast Red – Tartrazine Method

Muscle and Bones

Schmorl’s Picro Thionine Method

Bones: Lacunae, Canaliculi,& Bone Matrix

Goldner’s Method

Bone Cells: Osteoblast and Osteoclast

Heidenhain’s Iron Hematoxylin

resistant to fading if the section is washed well in running tap water after differentiation to remove all traces of iron alum

Lissamine Fast Red-Tartrazine Method

Routine staining method of choice in bone histology

Schmorl’s Picro Thionine Method

staining may be intensified by adding one to two drops of conc. ammonia before use