ANTIBODIES EXTRAS

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6 Terms

1
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If you want highly repeatable tissue staining, what type of antibodies will be best?

Monoclonal antibodies

They only recognize a single epitope which creates low batch to batch variability and reproducible staining

2
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The making of NANOBODIES simple steps:

  1. Immunize a camelid. The camelid will produce heavy chain only antibodies. The variable regions will be the nanobodies

  1. Collect immune cells

  1. Construct a library

  1. Selection. Only the antibodies that bind to the antigen are retained.

  1. Screening and Sequencing. The antibodies are tested to find the best binders.

  1. Production. Nanobodies can be cloned to make more.

  1. Purification & validation. Nanobodies are purified by chromatography.

3
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What is the Fc fragment made up of?

Two heavy chains

4
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What portion of the antibody structure do secondary antibodies bind to?

The Fc fragment

5
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How many Fab fragments are there per antibody?

Two

6
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What is the Fab fragment made up of?

Two hypervariable regions