Lecture 16

Rumen Microbiota

The community of microorganisms residing in the rumen of ruminant animals, crucial for digestion.

Rumen Cannula

An invasive technique providing direct access to rumen contents for sampling.

Oro-Esophageal Tube

A less invasive method to collect rumen content without surgical intervention.

Rumenocentesis

A minimally invasive sampling technique that accesses rumen fluid.

Buccal Swabs

A non-invasive method used to collect microbial DNA from the oral cavity.

Exhaled-Breath Metabolites

Non-invasive sampling that reflects metabolic activity in the rumen.

Biological Variables

Factors such as species, genetics, age, and diet that impact microbiome composition.

Sample Preservation

Methods used to maintain the integrity of microbiome samples during storage.

Culturomics

A methodology focused on cultivating a wide variety of microorganisms for analysis.

Anaerobic Media

Specially prepared growth media that support the growth of anaerobic microorganisms.

Strict Anaerobes

Microorganisms that can only grow in the absence of oxygen.

Facultative Anaerobes

Microorganisms capable of growth in both oxygen-rich and oxygen-deprived environments.

Genomics-Informed Isolation

Technique involving genomics to identify and isolate specific target microorganisms.

Fluorescence-Activated Cell Sorting

A method to isolate target cells based on surface protein labeling.

Case Study: Nanoarchaeota

Examines the isolation and characterization of Nanopusillus acidilobi, showcasing genomics-informed techniques.