Histo Sem W2: Dehydration

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13 Terms

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dehydration

removal of water from the tissue after fixation and prior to wax impregnation

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dehydration

usually “alcohol“

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dehydration

done by immersing specimen and in ascending grades of alcohol

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30%

starts at __% for delicate specimen

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70%

__% for routine specimen

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ethanol

best

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methanol

blood smear, tissue films, smear preparation

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butanol

plants and animal biopsies

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dioxane (diethyl dioxide)

gaupner’s method

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dioxane (diethyl dioxide)

weisenberger’s method

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tetrahydrofuran

offensive odor

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tetrahydrofuran

may cause conjuctival irritation

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triethyl phosphate

soluble in water, benzene, chloroform, ether, acetone, and xylene. (oil + bergamot)